ABCMdb

PMID: 24733388

Gupta RP, Kueppers P, Hanekop N, Schmitt L
Generating symmetry in the asymmetric ATP-binding cassette (ABC) transporter Pdr5 from Saccharomyces cerevisiae.
J Biol Chem. 2014 May 30;289(22):15272-9. doi: 10.1074/jbc.M114.553065. Epub 2014 Apr 14., [PubMed]

Sentences

No. Mutations Sentence Comment

71 ABCB1 p.Glu1013Gly Mutation Direction Sequence, 5d15; to 3d15; P195S S GCTAGTCGTTTTAGGTAGATCAGGCTCTGGCAAAAC AS GTTTTGCCAGAGCCTGATCTACCTAAAACGACTAGC C199K S CGTTTTAGGTAGACCAGGCTCTGGCAAAACTACTTTATTAAAATCCATCTCTTC AS GAAGAGATGGATTTTAATAAAGTAGTTTTGCCAGAGC CTGGTCTACCTAAAACG N334E S GATCCAAATTTCAATGCTGGGATGAAGCTACAAGGG GTTTGGATTC AS GAATCCAAACCCCTTGTAGCTTCATCCCAGCATTGAA ATTTGGATC Y367H S CTGCCACAGTGGCCATCCATCAATGTTCTCAAGATG AS CATCTTGAGAACATTGATGGATGGCCACTGTGGCAG N1011S S GTTGTTGGTGTTGCTGGTGAAGGTTTATCTGTTGAAC AAAGAAAAAGATTAACCATTG AS CAATGGTTAATCTTTTTCTTTGTTCAACAGATAAACCT TCACCAGCAACACCAACAAC V1012G S GTTGCTGGTGAAGGTTTAAACGGTGAACAAAGAAAA AGATTAACC AS GGTTAATCTTTTTCTTTGTTCACCGTTTAAACCTTCAC CAGCAAC E1013G S GTTGCTGGTGAAGGTTTAAACGTTGGTCAAAGAAAAA GATTAACCATTGGTG AS CACCAATGGTTAATCTTTTTCTTTGACCAACGTTTAAA CCTTCACCAGCAAC Role of Degenerate Nucleotide-binding Site in Pdr5 MAY 30, 2014ߦVOLUME 289ߦNUMBER 22 JOURNAL OF BIOLOGICAL CHEMISTRY 15273 at SEMMELWEIS UNIV OF MEDICINE on December 12, 40 mM H2SO4. Login to comment 86 ABCB1 p.Glu1013Gly In the following, restoration of the consensus sequence in the Walker A motif (C199K) located in NBD1 and forming NBS1 is called the "Walker A" mutant; restoration of the consensus sequence in the Walker B motif (N334E; NBD1) in NBS1 is called the "Walker B" mutant; restoration of the consensus sequence of the H-loop (Y367H, NBD1) in NBS1 is called the "H-loop" mutant, and restoration of the consensus sequence of the C-loop (N1011S/V1012G/ E1013G, NBD2) in NBS1 is called the "C-loop" mutant. Login to comment 112 ABCB1 p.Glu1013Gly ABCB1 p.Glu1013Gly ABCB1 p.Glu1013Gly ABCB1 p.Glu1013Gly TABLE 2 List of mutations generated and used in this study Mutation Abbreviation Alteration Motif Domain P195S Pro in Walker A Walker A NBD1 C199K Walker A Cys in Walker A Walker A NBD1 N334E Walker B Asn in Walker B Walker B NBD1 Y367H H-loop Tyr in H-loop H-loop NBD1 N1011S, V1012G, E1013G C-loop Asn, Val, and Glu in C-loop C-loop NBD2 Y367H, N1011S, V1012G, E1013G HC-loop Tyr in H-loop H-loop NBD1/2 Asn, Val, and Glu in C-loop C-loop C199K AHC Cys in Walker A Walker A NBD1/2 Y367H Tyr in H-loop H-loop N1011S, V1012G, E1013G Asn, Val, and Glu in C-loop C-loop P195S ABHC Pro in Walker A Walker A NBD1/2 C199K Cys in Walker A Walker B N334E Asn in Walker B H-loop Y367H Tyr in H-loop C-loop N1011S, V1012G, E1013G Asn, Val, and Glu in C-loop Role of Degenerate Nucleotide-binding Site in Pdr5 MAY 30, 2014ߦVOLUME 289ߦNUMBER 22 JOURNAL OF BIOLOGICAL CHEMISTRY 15275 at SEMMELWEIS UNIV OF MEDICINE on December 12, that the apparent affinity of these mutants toward ATP has not drastically changed (Table 3). Login to comment