ABCMdb

PMID: 24711118

Guyot C, Hofstetter L, Stieger B
Differential effects of membrane cholesterol content on the transport activity of multidrug resistance-associated protein 2 (ABCC2) and of the bile salt export pump (ABCB11).
Mol Pharmacol. 2014 Jun;85(6):909-20. doi: 10.1124/mol.114.092262. Epub 2014 Apr 7., [PubMed]

Sentences

No. Mutations Sentence Comment

11 ABCB11 p.Glu297Gly ABCB11 p.Arg432Thr The transport activity of the BSEP mutants p.E297G and p.R432T increased at high cholesterol content but did not reach the capacity of normal BSEP. Login to comment 177 ABCB11 p.Glu297Gly ABCB11 p.Arg432Thr Finally, we studied the effect of cholesterol on two mutant forms of BSEP, p.E297G and p.R432T, found in patients with BSEP deficiency syndromes. Login to comment 262 ABCB11 p.Glu297Gly ABCB11 p.Arg432Thr (A, D, and G) Western blot analysis of p.444A variant (A), p.E297G (D), and p.R432T (G) mutant BSEP-expressing vesicles with and without cholesterol loading (30 mg protein per lane). Login to comment