PMID: 24312054

Gauthier C, Ozvegy-Laczka C, Szakacs G, Sarkadi B, Di Pietro A
ABCG2 is not able to catalyze glutathione efflux and does not contribute to GSH-dependent collateral sensitivity.
Front Pharmacol. 2013 Nov 7;4:138. doi: 10.3389/fphar.2013.00138. eCollection 2013., [PubMed]
Sentences
No. Mutations Sentence Comment
52 ABCG2 p.Lys86Met
X
ABCG2 p.Lys86Met 24312054:52:151
status: NEW
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 MEMBRANE PREPARATION For obtaining membrane vesicles insect cells were infected with recombinant baculoviruses containing the cDNA of wtABCG2 or ABCG2-K86M (Ozvegy-Laczka et al., 2005) or of ABCC1 (Bakos 3 - #3 Gauthier et al. ABCG2 inability to transport glutathione et al.,1996). Login to comment 114 ABCG2 p.Lys86Met
X
ABCG2 p.Lys86Met 24312054:114:225
status: NEW
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 There was a low level of GSH accumulation in the presence of ABCG2 observed without ATP, which was also observed in the presence of the selective ABCG2 inhibitor Ko143 (Allen et al., 2002), or when the catalytically inactive K86M ABCG2 mutant was expressed. Login to comment 134 ABCG2 p.Lys86Met
X
ABCG2 p.Lys86Met 24312054:134:143
status: NEW
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 ATP-dependent transport of 3H-methotrexate in 2 mM cholesterol-loaded insect-cell membranes expressing ABCG2 (either wild-type or the inactive K86M mutant) was measured for 10 min at 37e6;C. Login to comment