ABCMdb

PMID: 24130050

Hou H, Sun H, Lu P, Ge C, Zhang L, Li H, Zhao F, Tian H, Zhang L, Chen T, Yao M, Li J
Tunicamycin potentiates cisplatin anticancer efficacy through the DPAGT1/Akt/ABCG2 pathway in mouse Xenograft models of human hepatocellular carcinoma.
Mol Cancer Ther. 2013 Dec;12(12):2874-84. doi: 10.1158/1535-7163.MCT-13-0201. Epub 2013 Oct 15., [PubMed]

Sentences

No. Mutations Sentence Comment

63 ABCG2 p.Asn596Gln The Asn596-encoding codon AAT was converted to CAA to generate the N596Q variant of ABCG2 using the megaprimer PCR method as described previously (27). Login to comment 64 ABCG2 p.Asn596Gln The N596Q mutant primer is listed in Supplementary Table S2. Login to comment 88 ABCG2 p.Asn596Gln To examine the potential role of NLG in stabilizing and degrading the ABCG2 protein, we established the N596Q variant of human ABCG2, in which Asn596, the amino acid for NLG, was substituted by Gln596. Login to comment 89 ABCG2 p.Asn596Gln Both wild-type (WT) ABCG2 and the N596Q variant were stably transfected into Huh7 cells. Login to comment 90 ABCG2 p.Asn596Gln ABCG2 p.Asn596Gln A Western blot analysis revealed that the protein expression level of the human ABCG2 N596Q variant in Huh7 cells was much lower than that of WT ABCG2 (Fig. 1C), whereas the mRNA expression levels of the WT and N596Q variant ABCG2 were nearly identical (Fig. 1D). Login to comment 116 ABCG2 p.Asn596Gln The shift in the ABCG2 molecular weight was detected by Western blotting. C, wild-type ABCG2 and the ABCG2 N596Q variant were stably transfected into Huh7 cells. Login to comment 117 ABCG2 p.Asn596Gln ABCG2 expression was analyzed using Western blotting. D, ABCG2 mRNA levels were analyzed by real-time PCR with total RNA extracted from Huh7 cells expressing wild-type ABCG2 or the N596Q variant. Login to comment 190 ABCG2 p.Asn596Ala A previous report showed that NLG is not essential for routing ABCG2 to the plasma membrane because the N596A mutation had little effect on ABCG2 subcellular localization (28). Login to comment