ABCMdb

PMID: 23799215

Kamei H, Masuda S, Nakamura T, Oike F, Takada Y, Hamajima N
Association of transporter associated with antigen processing (TAP) gene polymorphisms in donors with acute cellular rejection in living donor liver transplantation.
J Gastrointestin Liver Dis. 2013 Jun;22(2):167-71., [PubMed]

Sentences

No. Mutations Sentence Comment

4 ABCB2 p.Asp697Gly ABCB3 p.Arg651Cys Methods: We examined 155 LDLTs treated at Nagoya University or Kyoto University from 2004 to 2009 and analyzed the gene polymorphisms of TAP-1 p.Ile333Val, TAP-1 p.Asp697Gly, TAP-2 p.Arg651Cys, and TAP-2 p.Gln687Stop. Login to comment 28 ABCB2 p.Asp697Gly ABCB3 p.Arg651Cys Genotyping Genomic DNA was extracted from peripheral blood samplesobtainedfromthedonors.IsolatedDNAwasusedfor genotyping of transporter associated with antigen processing 1 (TAP-1) p.Ile333Val (rs#1057141), TAP-1 p.Asp697Gly (rs#1135216), TAP-2 p.Arg651Cys (rs#4148876), and TAP-2 p.Gln687Stop (rs#241448). Login to comment 29 ABCB2 p.Asp697Gly ABCB3 p.Arg651Cys Genotype for TAP-1 p.Ile333Val, TAP-1 p.Asp697Gly, TAP-2 p.Arg651Cys, and TAP-2 p.Gln687Stop was performed using a PCR-restriction fragment length polymorphism (RFLP) method. Login to comment 45 ABCB2 p.Asp697Gly On the other hand, the Gly allele of TAP-1 p.Asp697Gly in the donors was found to have a suggestive association with ACR incidence (p=0.025, p<0.0125 considered significant) and the logistic regression analysis (adjusted for age, gender, and initial CNI) showed recipients who Table I. Login to comment 48 ABCB2 p.Asp697Gly ABCB3 p.Arg651Cys DISCUSSION We analyzed the associations of the gene polymorphisms TAP-1 p.Ile333Val, TAP-1 p.Asp697Gly, TAP-2 p.Arg651Cys and TAP-2 p.Gln687Stop in donors with early ACR in cases of LDLT. Login to comment 68 ABCB2 p.Asp697Gly The p.Asp697Gly polymorphism is also known as Asp637Gly as stated in previous publications [3, 5, 6, 9-11, 15]. Login to comment 70 ABCB2 p.Asp697Gly ABCB3 p.Arg651Cys Relationship between gene polymorphisms of donor and acute cellular rejection (ACR) Gene Polymorphisms rs# n n Rejection % p* TAP1 p.Ile333Val 1057141 0.523 Ile/Ile 124 35 28.2 Ile/Val 26 10 38.5 Val/Val 5 2 40.0 TAP1 p.Asp697Gly 1135216 0.025 Asp/Asp 124 31 25.0 Asp/Gly 27 14 51.6 Gly/Gly 4 2 50.0 TAP2 p.Arg651Cys 4148876 0.91 Arg/Arg 119 37 31.1 Arg/Cys 33 9 27.3 Cys/Cys 3 1 33.3 TAP2 p.Gln687Stop 241448 0.19 Gln/Gln 20 7 35.0 Gln/Stop 63 14 22.2 Stop/Stop 72 26 36.1 * P-value from a chi-square test with 2 degrees of freedom Table III. Login to comment