PMID: 23658020

Wen PC, Verhalen B, Wilkens S, Mchaourab HS, Tajkhorshid E
On the origin of large flexibility of P-glycoprotein in the inward-facing state.
J Biol Chem. 2013 Jun 28;288(26):19211-20. doi: 10.1074/jbc.M113.450114. Epub 2013 May 8., [PubMed]
Sentences
No. Mutations Sentence Comment
136 ABCB1 p.Gly185Val
X
ABCB1 p.Gly185Val 23658020:136:17
status: NEW
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 Interestingly, a G185V mutation in human Pgp (equivalent to Gly-181 of mouse Pgp, a glycine that bends helix TM3, supplemental Fig. S6) is well studied and shown to improve the coupling between ATP hydrolysis and transport (72). Login to comment 137 ABCB1 p.Gly346Cys
X
ABCB1 p.Gly346Cys 23658020:137:17
status: NEW
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 In addition, the G346C mutation in human Pgp (equivalent to Gly-342 of mouse Pgp, which causes a kink in helix TM6, supplemental Fig. S6) is FIGURE 4. Login to comment 147 ABCB1 p.Gly341Cys
X
ABCB1 p.Gly341Cys 23658020:147:263
status: NEW
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ABCB1 p.Gly360Cys
X
ABCB1 p.Gly360Cys 23658020:147:273
status: NEW
view ABCB1 p.Gly360Cys details
 The suggested hinge role played by Gly-346 of human Pgp can also explain why the ATPase activity can only be significantly reduced by a mutation at this particular position but not elsewhere along the helix TM6 (73) even when other glycines in TM6 were targeted (G341C and G360C) (73). Login to comment