ABCMdb

PMID: 22872754

Gelissen IC, Sharpe LJ, Sandoval C, Rao G, Kockx M, Kritharides L, Jessup W, Brown AJ
Protein kinase A modulates the activity of a major human isoform of ABCG1.
J Lipid Res. 2012 Oct;53(10):2133-40. doi: 10.1194/jlr.M028795. Epub 2012 Aug 7., [PubMed]

Sentences

No. Mutations Sentence Comment

137 ABCG1 p.Ser389Ala Comparison of ABCG1 mRNA expression among the lines showed that expression was similar among the lines, with the exception of the S389A mutant ,which had approximately half the mRNA expression compared with the other cell lines (see supplementary Fig. IIIA). Login to comment 139 ABCG1 p.Ser388Ala ABCG1 p.Ser390Ala HDL-induced cholesterol export from cells expressing ABCG1(+12)-S388A and ABCG1(+12)- S390A was similar to that of cholesterol export from wild-type ABCG1(+12) cells (Fig. 4). Login to comment 140 ABCG1 p.Ser388Ala ABCG1 p.Ser390Ala Furthermore, upon incubation with H89, cholesterol export from cells expressing the S388A or S390A mutants was increased to a level similar to that of the parental ABCG1(+12). Login to comment 141 ABCG1 p.Ser389Ala However, HDL-mediated cholesterol efflux from the S389A mutant was on average slightly higher than the parental line, despite having lower mRNA expression, whereas cholesterol export from this mutant was completely unaffected by H89 treatment (Fig. 4). Login to comment 145 ABCG1 p.Ser389Asp Figure 5A shows that basal cholesterol export from ABCG1(+12)-S389D- expressing cells was reduced compared with that of the wild-type ABCG1(+12), whereas incubation with H89 did not affect the cholesterol export capacity to HDL. Login to comment 177 ABCG1 p.Ser389Ala ABCG1 p.Ser388Ala ABCG1 p.Ser390Ala H89 significantly increased cholesterol export from cells expressing ABCG1(+12), S388A, and S390A (P < 0.05), but not ABCG1(afa;12) or S389A. Login to comment 178 ABCG1 p.Ser389Asp Fig. 5. Effect of H89 on ABCG1(+12)-S389D cholesterol export and protein levels. Login to comment 179 ABCG1 p.Ser389Asp A: Cholesterol export was measured as described inthelegendtoFig.2andMaterialsandMethodsfromABCG1(+12)- S389D-overexpressing cells and compared with the parental ABCG1(+12) cell line, with and without the addition of H89 (10 òe;M). Login to comment 180 ABCG1 p.Ser389Asp B: ABCG1(+12)-S389D-overexpressing cells were incubated with or without H89 in the presence of cycloheximide (10 òe;g/ml), as described in the legend to Fig. 4. Login to comment