ABCMdb

PMID: 22821996

Hampton TH, Ballok AE, Bomberger JM, Rutkowski MR, Barnaby R, Coutermarsh B, Conejo-Garcia JR, O'Toole GA, Stanton BA
Does the F508-CFTR mutation induce a proinflammatory response in human airway epithelial cells?
Am J Physiol Lung Cell Mol Physiol. 2012 Sep 15;303(6):L509-18. doi: 10.1152/ajplung.00226.2011. Epub 2012 Jul 20., [PubMed]

Sentences

No. Mutations Sentence Comment

28 ABCC7 p.Trp1282* For example, studies on IB3-1 (èc;F508/W1282X) and S9 cells (IB3-1 cells complemented with wt-CFTR) demonstrate that P. aeruginosa elicits a more robust increase in IL-8 production in IB3-1 CF cells than in wt-CFTR-corrected S9 cells (1, 10), whereas studies on other matched cell lines reveal that, when challenged with P. aeruginosa, CF cells actually produce less IL-8 than non-CF cells, contrary to the generally accepted view that CF mutations enhance the inflammatory response of airway epithelial cells to P. aeruginosa (16, 22, 31). Login to comment 32 ABCC7 p.Trp1282* For example, studies on IB3-1 (⌬F508/W1282X) and S9 cells (IB3-1 cells complemented with wt-CFTR) demonstrate that P. aeruginosa elicits a more robust increase in IL-8 production in IB3-1 CF cells than in wt-CFTR-corrected S9 cells (1, 10), whereas studies on other matched cell lines reveal that, when challenged with P. aeruginosa, CF cells actually produce less IL-8 than non-CF cells, contrary to the generally accepted view that CF mutations enhance the inflammatory response of airway epithelial cells to P. aeruginosa (16, 22, 31). Login to comment 97 ABCC7 p.Trp1282* This experiment involved IB3-1 cells, which have a relatively uncommon CF genotype, èc;F508/W1282X (41). Login to comment 101 ABCC7 p.Trp1282* This experiment involved IB3-1 cells, which have a relatively uncommon CF genotype, ⌬F508/W1282X (41). Login to comment 161 ABCC7 p.Trp1282* ABCC7 p.Trp1282* Reanalysis of microarray gene expression identifies a proinflammatory phenotype in IB3-1 cells (èc;F508/W1282X) compared with S9 cells (èc;F508/W1282X/wt-CFTR). Login to comment 165 ABCC7 p.Trp1282* ABCC7 p.Trp1282* Reanalysis of microarray gene expression identifies a proinflammatory phenotype in IB3-1 cells (⌬F508/W1282X) compared with S9 cells (⌬F508/W1282X/wt-CFTR). Login to comment 216 ABCC7 p.Trp1282* Second, the genotype of the cells is different (i.e., IB3-1 cells have the èc;F508/ W1282X genotype and CFBE cells are èc;F508/èc;F508). Login to comment 220 ABCC7 p.Trp1282* Second, the genotype of the cells is different (i.e., IB3-1 cells have the ⌬F508/ W1282X genotype and CFBE cells are ⌬F508/⌬F508). Login to comment 225 ABCC7 p.Trp1282* Gene array data from S9 (wt-CFTR) and IB3-1 (èc;F508/W1282X) cells exposed to P. aeruginosa. Login to comment 229 ABCC7 p.Trp1282* Gene array data from S9 (wt-CFTR) and IB3-1 (⌬F508/W1282X) cells exposed to P. aeruginosa. Login to comment