ABCMdb

PMID: 22607224

Ma CI, Beckstead JA, Thompson A, Hafiane A, Wang RH, Ryan RO, Kiss RS
Tweaking the cholesterol efflux capacity of reconstituted HDL.
Biochem Cell Biol. 2012 Oct;90(5):636-45. doi: 10.1139/o2012-015. Epub 2012 May 18., [PubMed]

Sentences

No. Mutations Sentence Comment

6 ABCA1 p.Trp590Ser Efflux to PC-containing rHDL was stimulated by transfection of a nonfunctional ABCA1 mutant (W590S), suggesting that binding to ABCA1 represents a competing interaction. Login to comment 17 ABCA1 p.Trp590Ser L`efflux de rHDL contenant de la PC était stimulé par la transfection d`un mutant d`ABCA1 non fonctionnel (W590S), suggérant que la liaison à ABCA1 représente une interaction compétitive. Login to comment 67 ABCA1 p.Trp590Ser BHK cells overexpressing ABCG1, ABCA1, and a nonfunctional mutant, W590S ABCA1, were generated using mifepristone-inducible GeneSwitch system as previously described (Vaughan and Oram 2005, 2006). Login to comment 113 ABCA1 p.Trp590Ser Thus, as a negative control, we included BHK cells that inducibly express a nonfunctional mutant ABCA1 (W590S). Login to comment 140 ABCA1 p.Trp590Ser Efflux assays were performed with the four rHDL (5 mg panel A; 10 mg panel B) in the different cholesterol loaded BHK cells: BHK cells with no expressed protein (WT); BHK cells expressing ABCA1 (ABCA1); BHK cells expressing a dominant negative mutant of ABCA1 (ABCA1 W590S); and BHK cells expressing ABCG1 (ABCG1). Login to comment 146 ABCA1 p.Trp590Ser Secondly, for WT-PC rHDL, a demonstrable increase in binding to ABCA1 and ABCG1-expressing cells was observed, with no increase in binding to cells expressing the nonfunctional W590S ABCA1, compared with control BHK cells. Login to comment 149 ABCA1 p.Trp590Ser Thirdly, for WT-SM rHDL, we observed a moderate decrease in binding to ABCA1-expressing cells, a moderate increase in binding to ABCA1 W590S-expressing cells, and a sizeable and significant increase in binding to ABCG1-expressing cells. Login to comment 170 ABCA1 p.Trp590Ser Binding assays were performed with the four radiolabeled rHDL (10 mg apoA-I protein) on BHK cells with no expressed protein (WT), BHK cells expressing ABCA1 (ABCA1), BHK cells expressing a nonfunctional mutant ABCA1 (ABCA1 W590S), and BHK cells expressing ABCG1 (ABCG1). Login to comment 198 ABCA1 p.Trp590Ser However, rHDL with SM does not interact with ABCA1 (compare the efflux and binding for each rHDL with ABCA1 and the nonfunctional ABCA1 W590S). Login to comment