ABCMdb

PMID: 22511347

Qin L, Tam SP, Deeley RG
Effect of multiple cysteine substitutions on the functionality of human multidrug resistance protein 1 expressed in human embryonic kidney 293 cells: identification of residues essential for function.
Drug Metab Dispos. 2012 Jul;40(7):1403-13. Epub 2012 Apr 16., [PubMed]

Sentences

No. Mutations Sentence Comment

64 ABCC1 p.Cys265Ala ABCC1 p.Cys265Ala ABCC1 p.Cys208Ala ABCC1 p.Cys208Ala ABCC1 p.Cys682Ala ABCC1 p.Cys682Ala ABCC1 p.Cys1479Ala ABCC1 p.Cys1479Ala ABCC1 p.Cys1439Ala ABCC1 p.Cys1439Ala ABCC1 p.Cys555Ala ABCC1 p.Cys555Ala ABCC1 p.Cys730Ala ABCC1 p.Cys730Ala ABCC1 p.Cys1423Ala ABCC1 p.Cys1423Ala ABCC1 p.Cys1047Ala ABCC1 p.Cys1047Ala ABCC1 p.Cys1105Ala ABCC1 p.Cys1105Ala ABCC1 p.Cys1205Ala ABCC1 p.Cys1205Ala ABCC1 p.Cys563Ala ABCC1 p.Cys563Ala ABCC1 p.Cys984Ala ABCC1 p.Cys984Ala ABCC1 p.Cys375Ala ABCC1 p.Cys375Ala ABCC1 p.Cys1299Ala ABCC1 p.Cys1299Ala ABCC1 p.Cys388Ala ABCC1 p.Cys388Ala ABCC1 p.Cys744Ala ABCC1 p.Cys744Ala ABCC1 p.Cys1209Ala ABCC1 p.Cys1209Ala Name Inserted (ϩ) or Deleted (-) Restriction Enzymes Primer Sequence (5Ј-3Јa 1 C208A N.A. CCCTAATCCCGCCCCAGAGTCCAG 2 C265A -BsmI GGAAGAAGGAAGCCGCCAAGACTAG 3 C375A -PstI GTCACTGCCGCCTTGCAGACCCTCG 4 C388A N.A. CTTCCACATCGCCTTCGTCAGTGG 5 C555A ϩSpoI/NruI CACCTGGGTCGCGACGCCCTTTCTG 6 C563A ϩBalI/MscI GGTGGCCTTGGCCACATTTGCCGTC 7 C682A ϩNarI CCAGGTGGGCGCCGGAAAGTCGTC 8 C730A ϩEspI, SacI ATCCTTTTTGGAGCTCAGCTGGAGG 9 C744A -StuI GATACAGGCCGCTGCCCTCCTCC 10 C984A ϩBalI/MscI TTCCTTTTCATGGCCAACCATGTGTCC 11 C1047A ϩSacI/SstI TTGGCTTCCCGAGCTCTGCACGTGG 12 C1105A ϩPvuI CATTGGTGCCGCGATCGTTATCCTG 13 C1205A N.A. GCGGCTGGAGGCTGTGGGCAACTG 14 C1209A ϩPvuI GTGTGGGCAACGCGATCGTTCTGTTTG 15 C1299A ϩMluI TTCCGGAACTACGCGTTGCGCTACCGAG 16 C1423A ϩPstI CTAGACCATGAAGCTGCAGAAGGC 17 C1439A ϩPflMI CCAGCTTGTGGCCCTAGCCCGGG 18 C1479A N.A. GTTCGAGGACGCCACCGTCCTCAC 19 Rec L N.A. GAAACCATCCACGACCCTAATCCCGCCCCAGAG 20 Rec R N.A. GGATTAGGGTCGTGGATGGTTTCCGAGAACAG 21 KbnL N.A. CATGGTACCATGGCGCTCCGGGGCTTCTGCAGC 22 KbnR N.A. GGCAGGATCCTTGGAGGAGTACACAACCTTC N.A., not applicable. Login to comment 190 ABCC1 p.Cys265Ala ABCC1 p.Cys208Ala The activity of èc;MRP1204-282cysless containing the C208A and C265A mutations was most dramatically affected and was decreased by b03;70% (Fig. 5B, top panel). Login to comment 191 ABCC1 p.Cys265Ala ABCC1 p.Cys265Ala ABCC1 p.Cys208Ala ABCC1 p.Cys208Ala The activity of ⌬MRP1204-282cysless containing the C208A and C265A mutations was most dramatically affected and was decreased by ϳ70% (Fig. 5B, top panel). Login to comment 192 ABCC1 p.Cys265Ala ABCC1 p.Cys208Ala Subsequently, we found that LTC4 transport activity of the C208A and C265A double mutant could be substantially recovered by growth of the HEK transfectants at 28°C (Fig. 5B, bottom panel). Login to comment 331 ABCC1 p.Cys388Ser ABCC1 p.Cys388Val ABCC1 p.Cys388Val ABCC1 p.Cys388Ile ABCC1 p.Cys388Asn ABCC1 p.Cys388Asn ABCC1 p.Cys388Met ABCC1 p.Cys388Gly ABCC1 p.Cys388Asp Substitution of Cys388 with seven different amino acids (C388D, C388G, C388I, C388M, C388N, C388S, and C388V) decreased the transport of LTC4 by 30% (C388V) to more than 90% (C388N). Login to comment 332 ABCC1 p.Cys388Ser ABCC1 p.Cys388Val ABCC1 p.Cys388Val ABCC1 p.Cys388Val ABCC1 p.Cys388Ile ABCC1 p.Cys388Asn ABCC1 p.Cys388Asn ABCC1 p.Cys388Met ABCC1 p.Cys388Gly ABCC1 p.Cys388Asp Their effect on E217betaG transport was similar although the C388V mutation reduced transport by only 10 to 15% (data not shown). Login to comment 333 ABCC1 p.Cys388Val Their effect on E217betaG transport was similar although the C388V mutation reduced transport by only 10 to 15% (data not shown). Login to comment