ABCMdb

PMID: 22310978

Ji M, Tang J, Zhao J, Xu B, Qin J, Lu J
Polymorphisms in genes involved in drug detoxification and clinical outcomes of anthracycline-based neoadjuvant chemotherapy in Chinese Han breast cancer patients.
Cancer Biol Ther. 2012 Mar;13(5):264-71. doi: 10.4161/cbt.18920. Epub 2012 Mar 1., [PubMed]

Sentences

No. Mutations Sentence Comment

17 ABCB1 p.Ala313Gly The polymorphism of GSTP1 A313G and null variants of GSTM1 and GSTT1 that lead to the diminished or abolished enzyme activity have been associated with increased drug treatment benefit in some cancer patients.5,7 In addition to these enzymes, drug transporters are increasing recognized to be important to drug disposition and response. Login to comment 48 ABCB1 p.Ala313Gly The combined effects of GSTP1 A313G and MDR1 C3435T were greater than those for either polymorphism alone, and an increased response with decreasing number of adverse genotypes was observed (14.3 vs. 66.0 vs. 82.2%; p = 0.000). Login to comment 84 ABCB1 p.Ala313Gly The combined adverse genotypes in GSTP1 A313G and MDR1 C3435T and clinical response to anthracycline-based chemotherapy Adverse genotype No. Clinical response x2 p RR (CI 95%)a CR + PR (%) SD + PD (%) GSTP1 313AA and MDR1 3435TT 0 45 37 (82.2) 5 (17.8) 26.223 0.000 1.0 (Ref.) 1 (AA or TT) 94 62 (66.0) 32 (34.0) 3.82 (1.37-10.66) 2 (AA + TT) 14 2 (14.3) 12 (85.7) 44.40 (7.61-259.20) a The relative risk (RR) of not responding to treatment with a 95% confidence interval. Login to comment 102 ABCB1 p.Ala313Gly Volume 13 Issue 5 Single nucleotide polymorphisms (SNPs) of MnSOD (T47C), CAT (C-262T), GSTP1 (A313G) and the deletion polymorphisms of GSTM1 and GSTT1 were determined using allele-specific oligonucleotide ligation reaction (ASOLR) and a multiplex PCR developed in our laboratory, respectively.28 ASOLR was a technique for multiplex genetic typing. Login to comment 103 ABCB1 p.Ala313Gly The assay was implemented via three steps: a triplex PCR that resulted in the amplified target sequences with MnSOD T47C, CAT C-262T and GSTP1 A313G loci, a triplex allele-specific oligonucleotide probe ligation reaction that generates the ligation between perfectly matched probes at their junctions while no ligation occurred between mismatched ones and an analysis of genotype-specific ligation products by both fluorescence and size after electrophoresis on ABI PRISM 377 DNA sequencer.28,29 MDR1 C3435T, G2677T/A and C1236T polymorphisms were analyzed by PCR-RFLP as described by Ni et al. PCR reaction was performed on a DNA thermal cycler (Bio-Rad iCycle). Login to comment