PMID: 22119192

Kim J, Yoon H, Ramirez CM, Lee SM, Hoe HS, Fernandez-Hernando C, Kim J
MiR-106b impairs cholesterol efflux and increases Abeta levels by repressing ABCA1 expression.
Exp Neurol. 2012 Jun;235(2):476-83. Epub 2011 Nov 18., [PubMed]
Sentences
No. Mutations Sentence Comment
41 ABCA1 p.Met671Leu
X
ABCA1 p.Met671Leu 22119192:41:115
status: NEW
view ABCA1 p.Met671Leu details
 pcDNA3.1- mouse ABCA1 open reading frame (ORF) without 3' untranslated region (UTR) and pAG3-Swedish mutant (K670N/M671L) APPsw ORF without 3' UTR were kind gifts from Dr. David M. Holtzman (Washington University, St. Louis, USA) and Dr. Todd Golde (University of Florida, Gainesville, USA), respectively. Login to comment 70 ABCA1 p.Met671Leu
X
ABCA1 p.Met671Leu 22119192:70:235
status: NEW
view ABCA1 p.Met671Leu details
ABCA1 p.Met671Leu
X
ABCA1 p.Met671Leu 22119192:70:238
status: NEW
view ABCA1 p.Met671Leu details
 Measurement of secreted Aβ levels Neuro2a cells were plated at a density of 1.25×105 cells/well in a 24-well plate 1 day before transfection. Neuro2A cells were first transfected with 0.3 μg of pAG3-Swedish mutant (K670N/M671L) APPsw ORF without 3' UTR for 8 h and allowed to recover overnight. Then, miR-106b or a scrambled negative control was transfected into cells. Login to comment