PMID: 22104130

Kato T, Hamada A, Mori S, Saito H
Genetic polymorphisms in metabolic and cellular transport pathway of methotrexate impact clinical outcome of methotrexate monotherapy in Japanese patients with rheumatoid arthritis.
Drug Metab Pharmacokinet. 2012 Apr 25;27(2):192-9. Epub 2011 Nov 22., [PubMed]
Sentences
No. Mutations Sentence Comment
56 ABCB1 p.Ala80Gly
X
ABCB1 p.Ala80Gly 22104130:56:86
status: NEW
view ABCB1 p.Ala80Gly details
 To determine the genotype at the RFC1 80AhG polymorphism, the region encompassing the A80G polymorphic site was amplified ¤forward primer: 5$-AGCGGTGGAGAAGCAGGT-3$, reverse primer: 5$-GGAGGTAGGGGGTGATGAAG-3$¥, and the PCR products were digested with HhaI ¤New England BioLabs, Tokyo, Japan¥ as previously described.24¥ To determine the genotype at the ABCB1 2677GhA/T polymorphism, the region encompassing the 2677GhA/T polymorphic site was amplified by PCR ¤forward primer: 5$-TCTTAGCAATTGTACCCATCATTG-3$, reverse primer: 5$-CAGGTTCTTGACCGAAACGA-3$¥, and the resulting PCR products were analyzed by direct sequencing. Login to comment