PMID: 21949127

Thevenot E, Moreau AW, Rousseau V, Combeau G, Domenichini F, Jacquet C, Goupille O, Amar M, Kreis P, Fossier P, Barnier JV
p21-Activated kinase 3 (PAK3) protein regulates synaptic transmission through its interaction with the Nck2/Grb4 protein adaptor.
J Biol Chem. 2011 Nov 18;286(46):40044-59. Epub 2011 Sep 23., [PubMed]
Sentences
No. Mutations Sentence Comment
48 ABCC8 p.Ala365Glu
X
ABCC8 p.Ala365Glu 21949127:48:101
status: NEW
view ABCC8 p.Ala365Glu details
 The following plasmids were described previously (10, 24): the pcDNA3-HA-PAK3-WT, -kd, -ca, -R419X, -A365E, and -R67C plasmids encode mouse HA-tagged PAK3 wild-type, K297L kinase-defective, T421E constitutively active, mental retardation truncated mutant, missense kinase defective mutant, and the missense R67C mutated proteins, respectively. Login to comment 183 ABCC8 p.Ala365Glu
X
ABCC8 p.Ala365Glu 21949127:183:62
status: NEW
view ABCC8 p.Ala365Glu details
 We also tested the capacity of the mental retardation mutated A365E and R419X PAK3 proteins to interact with Nck2 in co-immunoprecipitation assays, and no difference was observed between these mutated proteins compared with the wild type (Fig. 2D). Login to comment 231 ABCC8 p.Ala365Glu
X
ABCC8 p.Ala365Glu 21949127:231:63
status: NEW
view ABCC8 p.Ala365Glu details
 D, three PAK3 proteins bearing the mental retardation mutation A365E, R419Stop, and R67C co-immunoprecipitate with Nck2 with an apparent normal efficiency. Login to comment