ABCMdb

PMID: 21926174

Bedekovics T, Li H, Gajdos GB, Isaya G
Leucine biosynthesis regulates cytoplasmic iron-sulfur enzyme biogenesis in an Atm1p-independent manner.
J Biol Chem. 2011 Nov 25;286(47):40878-88. Epub 2011 Sep 16., [PubMed]

Sentences

No. Mutations Sentence Comment

95 ABCB7 p.Leu144Ala We selected point mutations in Yfh1p (Y73A, L144A, and V150A) that did not result in obvious phenotypes when yeast cells grew logarithmically in rich medium (Ref. 21 and data not shown). Login to comment 138 ABCB7 p.Leu144Ala ABCB7 p.Leu144Ala ABCB7 p.Leu144Ala ABCB7 p.Leu144Ala ABCB7 p.Leu144Ala ABCB7 p.Leu144Ala YFH1leu2⌬MAT␣ura3-52lys2-801amber ade2-101ochre trp1-⌬63his3-⌬200leu2-⌬1yfh1⌬::HIS3ϩYCplac22-YFH1-TRP1[␳ϩ ]21 yfh1Y73A leu2⌬MAT␣ura3-52lys2-801amber ade2-101ochre trp1-⌬63his3-⌬200leu2-⌬1yfh1⌬::HIS3ϩpRSGPD-YFH1[Y73A][␳ϩ ]21 yfh1L144A leu2⌬MAT␣ura3-52lys2-801amber ade2-101ochre trp1-⌬63his3-⌬200leu2-⌬1yfh1⌬::HIS3ϩpRSGPD-YFH1[L144A][␳ϩ ]Thisstudy yfh1L144A LEU2(2␮m)MAT423;ura3-52lys2-801amber ade2-101ochre trp1-⌬63his3-⌬200leu2-⌬1yfh1⌬::HIS3ϩpRSGPD-YFH1[L144A][␳ϩ ]ϩYep24-LEU2-URA3Thisstudy yfh1V150A leu2⌬MAT␣ura3-52lys2-801amber ade2-101ochre trp1-⌬63his3-⌬200leu2-⌬1yfh1⌬::HIS3ϩpRSGPD-YFH1[V150A][␳ϩ ]Thisstudy yfh1V150A LEU2(2␮m)MAT␣ura3-52lys2-801amber ade2-101ochre trp1-⌬63his3-⌬200leu2-⌬1yfh1⌬::HIS3ϩpRSGPD-YFH1[V150A][␳ϩ ]ϩYep24-LEU2-URA3Thisstudy YFH1LEU2MAT␣ura3-52lys2-801amber ade2-101ochre trp1-⌬63his3-⌬200yfh1⌬::HIS3ϩYCplac22-YFH1-TRP1[␳ϩ ]Thisstudy yfh1Y73A LEU2MAT␣ura3-52lys2-801amber ade2-101ochre trp1-⌬63his3-⌬200yfh1⌬::HIS3ϩpRSGPD-YFH1[Y73A][␳ϩ ]Thisstudy yfh1Y73A LEU2(CEN)MAT␣ura3-52lys2-801amber ade2-101ochre trp1-⌬63his3-⌬200yfh1⌬::HIS3ϩpRSGPD-YFH1[Y73A][␳ϩ ]ϩpRS415-LEU2Thisstudy yfh1Y73A LEU2(2␮m)MAT␣ura3-52lys2-801amber ade2-101ochre trp1-⌬63his3-⌬200yfh1⌬::HIS3ϩpRSGPD-YFH1[Y73A][␳ϩ ]ϩYep24-LEU2-URA3Thisstudy YFH1LEU1-FLAGLEU2-FLAGMAT␣ura3-52lys2-801amber ade2-101ochre trp1-⌬63his3-⌬200yfh1⌬::HIS3ϩYCplac22-YFH1-TRP1[␳ϩ ]LEU1-FLAGLEU2-FLAGThisstudy yfh1Y73A LEU1-FLAGLEU2-FLAGMAT␣ura3-52lys2-801amber ade2-101ochre trp1-⌬63his3-⌬200yfh1⌬::HIS3ϩpRSGPD-YFH1[Y73A][␳ϩ ]LEU1-FLAGLEU2-FLAGThisstudy YFH1leu1⌬LEU2-FLAGMAT␣ura3-52lys2-801amber ade2-101ochre trp1-⌬63his3-⌬200yfh1⌬::HIS3ϩYCplac22-YFH1-TRP1[␳ϩ ]leu1⌬::KanMX4LEU2-FLAGThisstudy yfh1Y73A leu1⌬LEU2(CEN)MAT␣ura3-52lys2-801amber ade2-101ochre trp1-⌬63his3-⌬200yfh1⌬::HIS3ϩpRSGPD-YFH1[Y73A][␳ϩ ]leu1⌬::KanMX4ϩpRS415-LEU2Thisstudy yfh1Y73A leu1⌬LEU2(2␮m)MAT␣ura3-52lys2-801amber ade2-101ochre trp1-⌬63his3-⌬200yfh1⌬::HIS3ϩpRSGPD-YFH1[Y73A][␳ϩ ]leu1⌬::KanMX4ϩYep24-LEU2-URA3Thisstudy yfh1Y73A leu1⌬LEU2-FLAGMAT␣ura3-52lys2-801amber ade2-101ochre trp1-⌬63his3-⌬200yfh1⌬::HIS3ϩpRSGPD-YFH1[Y73A][␳ϩ ]leu1⌬::KanMX4LEU2-FLAGThisstudy YFH1LEU1-FLAGleu2⌬MAT␣ura3-52lys2-801amber ade2-101ochre trp1-⌬63his3-⌬200yfh1⌬::HIS3ϩYCplac22-YFH1-TRP1[␳ϩ ]LEU1-FLAGleu2⌬::KanMX4Thisstudy yfh1Y73A LEU1-FLAGleu2⌬MAT␣ura3-52lys2-801amber ade2-101ochre trp1-⌬63his3-⌬200yfh1⌬::HIS3ϩpRSGPD-YFH1[Y73A][␳ϩ ]LEU1-FLAGleu2⌬::KanMX4Thisstudy YFH1LEU1-FLAGLEU2-FLAGleu3⌬MAT␣ura3-52lys2-801amber ade2-101ochre trp1-⌬63his3-⌬200yfh1⌬::HIS3ϩYCplac22-YFH1-TRP1[␳ϩ ]LEU1-FLAGLEU2-FLAGleu3⌬::URA3Thisstudy yfh1Y73A LEU1-FLAGLEU2-FLAGleu3⌬MAT␣ura3-52lys2-801amber ade2-101ochre trp1-⌬63his3-⌬200yfh1⌬::HIS3ϩpRSGPD-YFH1[Y73A][␳ϩ ]LEU1-FLAGLEU2-FLAGleu3⌬::URA3Thisstudy YFH1leu1⌬-LEU2-FLAGleu3⌬MAT␣ura3-52lys2-801amber ade2-101ochre trp1-⌬63his3-⌬200yfh1⌬::HIS3ϩYCplac22-YFH1-TRP1[␳ϩ ]leu1⌬::KanMX4LEU2-FLAGleu3⌬::URA3Thisstudy yfh1Y73A leu1⌬-LEU2-FLAGleu3⌬MAT␣ura3-52lys2-801amber ade2-101ochre trp1-⌬63his3-⌬200yfh1⌬::HIS3ϩpRSGPD-YFH1[Y73A][␳ϩ ]leu1⌬::KanMX4LEU2-FLAGleu3⌬::URA3Thisstudy YFH1LEU1-FLAGleu2⌬leu3⌬MAT␣ura3-52lys2-801amber ade2-101ochre trp1-⌬63his3-⌬200yfh1⌬::HIS3ϩYCplac22-YFH1-TRP1[␳ϩ ]LEU1-FLAGleu2⌬::KanMX4leu3⌬::URA3Thisstudy yfh1Y73A LEU1-FLAGleu2⌬leu3⌬MAT␣ura3-52lys2-801amber ade2-101ochre trp1-⌬63his3-⌬200yfh1⌬::HIS3ϩpRSGPD-YFH1[Y73A][␳ϩ ]LEU1-FLAGleu2⌬::KanMX4leu3⌬::URA3Thisstudy YFH1leu1⌬LEU2-FLAGleu3⌬MAT␣ura3-52lys2-801amber ade2-101ochre trp1-⌬63his3-⌬200yfh1⌬::HIS3ϩYCplac22-YFH1[Y73A]-TRP1[␳ϩ ]leu1⌬::KanMX4LEU2-FLAGleu3⌬::URA3Thisstudy yfh1Y73A leu1⌬LEU2-FLAGleu3⌬MAT␣ura3-52lys2-801amber ade2-101ochre trp1-⌬63his3-⌬200yfh1⌬::HIS3ϩpRSGPD-YFH1[Y73A][␳ϩ ]leu1⌬::KanMX4LEU2-FLAGleu3⌬::URA3Thisstudy yfh1Y73A leu2⌬aco1⌬MAT␣ura3-52lys2-801amber ade2-101ochre trp1-⌬63his3-⌬200leu2-⌬1yfh1⌬::HIS3ϩpRSGPD-YFH1[Y73A][␳ϩ ]Aco1⌬::KanMX4Thisstudy yfh1Y73 LEU2aco1⌬MAT␣ura3-52lys2-801amber ade2-101ochre trp1-⌬63his3-⌬200yfh1⌬::HIS3ϩYCplac22-YFH1[Y73A]-TRP1[␳ϩ ]Aco1⌬::KanMX4Thisstudy LEU2ATM1MAT␣ura3-52lys2-801amber ade2-101ochre trp1-⌬63his3-⌬200yfh1⌬::HIS3ϩYCplac22-YFH1-TRP1[␳ϩ ]ATM1-FLAG3-URA3Thisstudy leu2⌬ATM1MAT␣ura3-52lys2-801amber ade2-101ochre trp1-⌬63his3-⌬200leu2-⌬1yfh1⌬::HIS3ϩYCplac22-YFH1-TRP1[␳ϩ ]ATM1-FLAG3-URA3Thisstudy LEU2Gal-ATM1MAT␣ura3-52lys2-801amber ade2-101ochre trp1-⌬63his3-⌬200yfh1⌬::HIS3ϩYCplac22-YFH1-TRP1[␳ϩ ]KanMX4-Gal-ATM1-FLAG3-URA3Thisstudy leu2⌬Gal-ATM1MAT␣ura3-52lys2-801amber ade2-101ochre trp1-⌬63his3-⌬200leu2-⌬1yfh1⌬::HIS3ϩYCplac22-YFH1-TRP1[␳ϩ ]KanMX4-Gal-ATM1-FLAG3-URA3Thisstudy LEU1-FLAGLEU2-FLAGATM1MAT␣ura3-52lys2-801amber ade2-101ochre trp1-⌬63his3-⌬200yfh1⌬::HIS3ϩYCplac22-YFH1-TRP1[␳ϩ ]LEU1-FLAGLEU2-FLAGATM1-FLAG3-URA3Thisstudy LEU1-FLAGleu2⌬ATM1MAT␣ura3-52lys2-801amber ade2-101ochre trp1-⌬63his3-⌬200leu2-⌬1yfh1⌬::HIS3ϩYCplac22-YFH1-TRP1[␳ϩ ]LEU1-FLAGATM1-FLAG3-URA3Thisstudy LEU1-FLAGLEU2-FLAGGal-ATM1MAT␣ura3-52lys2-801amber ade2-101ochre trp1-⌬63his3-⌬200yfh1⌬::HIS3ϩYCplac22-YFH1-TRP1[␳ϩ ]LEU1-FLAGLEU2-FLAGKanMX4-Gal-ATM1-FLAG3-URA3Thisstudy LEU1-FLAGleu2⌬Gal-ATM1MAT␣ura3-52lys2-801amber ade2-101ochre trp1-⌬63his3-⌬200leu2-⌬1yfh1⌬::HIS3ϩYCplac22-YFH1-TRP1[␳ϩ ]LEU1-FLAGKanMX4-Gal-ATM1-FLAG3-URA3Thisstudy Leucine Synthesis Controls ISC Assembly 40882 cally active Leu1p. 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