ABCMdb

PMID: 21846716

Iatan I, Bailey D, Ruel I, Hafiane A, Campbell S, Krimbou L, Genest J
Membrane microdomains modulate oligomeric ABCA1 function: impact on apoAI-mediated lipid removal and phosphatidylcholine biosynthesis.
J Lipid Res. 2011 Nov;52(11):2043-55. Epub 2011 Aug 16., [PubMed]

Sentences

No. Mutations Sentence Comment

64 ABCA1 p.Gln597Arg Fibroblasts obtained from patients with Tangier disease (TD; homozygous for Q597R at the ABCA1 gene) were used as a negative control throughout the study as previously described (9, 17). Login to comment 65 ABCA1 p.Gln597Arg Fibroblasts obtained from patients with Tangier disease (TD; homozygous for Q597R at the ABCA1 gene) were used as a negative control throughout the study as previously described (9, 17). Login to comment 95 ABCA1 p.Gln597Arg ABCA1 mutant (Q597R) associated with TD was used as a negative control for binding specificity. Login to comment 96 ABCA1 p.Gln597Arg ABCA1 mutant (Q597R) associated with TD was used as a negative control for binding specificity. Login to comment 125 ABCA1 p.Gln597Arg Normal and ABCA1 mutant (Q597R) human fibroblasts (B-D) and THP-1 cells (E-G) stimulated or not with 22OH/9CRA were incubated with 10 ␮g/ml [ 125 I]apoA-I for 45 min at 37°C. After washing to remove unbound [ 125 I] apoA-I, cross-linking with DSP was performed as described in Experimental Procedures. Login to comment 128 ABCA1 p.Gln597Arg Normal and ABCA1 mutant (Q597R) human fibroblasts (B-D) and THP-1 cells (E-G) stimulated or not with 22OH/9CRA were incubated with 10 g/ml [ 125 I]apoA-I for 45 min at 37&#b0;C. After washing to remove unbound [ 125 I] apoA-I, cross-linking with DSP was performed as described in Experimental Procedures. Login to comment 151 ABCA1 p.Gln597Arg An ABCA1 mutant (Q597R) used as a negative control for binding specificity did not show significant [125 I]apoA-I association with any sucrose fraction (Fig. 1B). Login to comment 155 ABCA1 p.Gln597Arg An ABCA1 mutant (Q597R) used as a negative control for binding specificity did not show significant [125 I]apoA-I association with any sucrose fraction (Fig. 1B). Login to comment 212 ABCA1 p.Gln597Arg An ABCA1 mutant (Q597R) that has been shown to have no significant apoA-I binding was used as a negative control. Login to comment 216 ABCA1 p.Gln597Arg An ABCA1 mutant (Q597R) that has been shown to have no significant apoA-I binding was used as a negative control. Login to comment 227 ABCA1 p.Gln597Arg Incubation with apoA-I did not upregulate mRNA expression of CCT␣ or CK␣ in ABCA1 mutant (Q597R) fibroblasts (Fig. 5D). Login to comment 232 ABCA1 p.Gln597Arg Incubation with apoA-I did not upregulate mRNA expression of CCT or CK in ABCA1 mutant (Q597R) fibroblasts (Fig. 5D). Login to comment 252 ABCA1 p.Gln597Arg (A, B) Normal and ABCA1 mutant (Q597R) fibroblasts were stimulated with 22OH/9CRA and incubated or not with 20 ␮g/ml apoA-I for 16 h. Cells were then pulsed with 10 ␮Ci/ml methyl[ 3 H]choline for 1 h, and the distribution of radioactivity among metabolites (Cho, Pcho, and CDP-cho) and PtdCho was determined as described in Experimental Procedures. Values shown are means ± SD of triplicate measures. Login to comment 254 ABCA1 p.Gln597Arg (C, D) Stimulated normal and ABCA1 mutant (Q597R) fibroblasts were incubated or not with 20 ␮g/ml apoA-I for 16 h, followed by mRNA extraction using the RNeasy mini RNA extraction kit (Qiagen). Login to comment 257 ABCA1 p.Gln597Arg (A, B) Normal and ABCA1 mutant (Q597R) fibroblasts were stimulated with 22OH/9CRA and incubated or not with 20 g/ml apoA-I for 16 h. Cells were then pulsed with 10 Ci/ml methyl[ 3 H]choline for 1 h, and the distribution of radioactivity among metabolites (Cho, Pcho, and CDP-cho) and PtdCho was determined as described in Experimental Procedures. Values shown are means &#b1; SD of triplicate measures. Login to comment 259 ABCA1 p.Gln597Arg (C, D) Stimulated normal and ABCA1 mutant (Q597R) fibroblasts were incubated or not with 20 g/ml apoA-I for 16 h, followed by mRNA extraction using the RNeasy mini RNA extraction kit (Qiagen). Login to comment 270 ABCA1 p.Gln597Arg (Top) Normal and ABCA1 mutant (Q597R) fibroblasts were stimulated with 22OH/9CRA and incubated in the presence of 10 ␮g/ml of [ 125 I]apoA-I for 45 min at 37°C. After washing to remove unbound [ 125 I]apoAI, DMEM was added, and plates were immediately incubated at 37°C or 4°C. Login to comment 275 ABCA1 p.Gln597Arg (Top) Normal and ABCA1 mutant (Q597R) fibroblasts were stimulated with 22OH/9CRA and incubated in the presence of 10 g/ml of [ 125 I]apoA-I for 45 min at 37&#b0;C. After washing to remove unbound [ 125 I]apoAI, DMEM was added, and plates were immediately incubated at 37&#b0;C or 4&#b0;C. Login to comment