ABCMdb

PMID: 21778412

Parker D, Cohen TS, Alhede M, Harfenist BS, Martin FJ, Prince A
Induction of Type I Interferon Signaling by Pseudomonas aeruginosa is Diminished in Cystic Fibrosis Epithelial Cells.
Am J Respir Cell Mol Biol. 2011 Jul 21., 2011-07-21 [PubMed]

Sentences

No. Mutations Sentence Comment

41 ABCC7 p.Trp1282* ABCC7 p.Trp1282* Materials and methods Cell culture IB3 (∆F508/ W1282X) or C38 (corrected) cystic fibrosis epithelial cell lines were grown between passage 22 and 60 in LHC-8 media, 10% fetal bovine serum, penicillin, streptomycin and L-glutamine. Login to comment 81 ABCC7 p.Trp1282* Decreased type I IFN responses in cells with CFTR mutations - To determine if mutations in CFTR affect induction of the type I IFN cascade, we compared the effects 5 of P. aeruginosa or LPS on the Ifnb expression in the matched C38 (corrected) and IB3 (∆F508/W1282X) (containing the episomal vector) cell lines (41) (Figure 3A&B). Login to comment 92 ABCC7 p.Trp1282* (A) Confluent monolayers of IB3 (ΔF508/ W1282X) or C38 (corrected) epithelial cell lines were infected with P. aeruginosa (P.a.; 107 CFUs; n ¼ 3) or LPS (n ¼ 2) for 2 or 4 hours, and concentrations of Ifnb were quantified by quantitaive RT-PCR. Login to comment 116 ABCC7 p.Trp1282* Decreased Type I IFN Responses in Cells with CFTR Mutations To determine if mutations in CFTR affect the induction of the type I IFN cascade, we compared the effects of P. aeruginosa or LPS on the expression of Ifnb in matched C38 (corrected) and IB3 (DF508/W1282X, containing the episomal vector) cell lines (39) (Figures 3A and 3B). Login to comment 128 ABCC7 p.Trp1282* Using data obtained from well characterized CF (∆F508/W1282X) plus vector and corrected cell lines we suggest that CFTR mutation may affect epithelial induction of IFN-β expression by airway cells in response to P. aeruginosa, the most proximal step in the mucosal response to inhaled airway pathogens. Login to comment 185 ABCC7 p.Trp1282* Using data obtained from well-characterized CF (DF508/ W1282X) plus vector and corrected cell lines, we suggest that CFTR mutations may affect the epithelial induction of IFN-b expression by airway cells in response to P. aeruginosa, the most proximal step in the mucosal response to inhaled airway pathogens. Login to comment