ABCMdb

PMID: 21697542

Lee JY, Karwatsky J, Ma L, Zha X
ABCA1 increases extracellular ATP to mediate cholesterol efflux to ApoA-I.
Am J Physiol Cell Physiol. 2011 Oct;301(4):C886-94. Epub 2011 Jun 22., [PubMed]

Sentences

No. Mutations Sentence Comment

7 ABCA1 p.Ala937Val Importantly, a dysfunctional ABCA1 mutant (A937V), when expressed similarly as wild-type ABCA1, is unable to raise extracellular ATP concentration, which suggests a casual relationship between functional ABCA1 and elevated extracellular ATP. Login to comment 11 ABCA1 p.Ala937Val Importantly, a dysfunctional ABCA1 mutant (A937V), when expressed similarly as wild-type ABCA1, is unable to raise extracellular ATP concentration, which suggests a casual relationship between functional ABCA1 and elevated extracellular ATP. Login to comment 58 ABCA1 p.Ala937Val BHK-A937V cells contain an ABCA1 gene insert carrying a single nucleotide A to V mutation. Login to comment 61 ABCA1 p.Ala937Val BHK-A937V cells contain an ABCA1 gene insert carrying a single nucleotide A to V mutation. Login to comment 112 ABCA1 p.Ala937Val RESULTS To determine whether functional ABCA1 influences extracellular ATP levels, we analyzed ATP concentrations in the medium of BHK-ABCA1 cells and compared them with the medium collected from Mock-BHK or BHK-A937V cells. Login to comment 114 ABCA1 p.Ala937Val Specifically, BHK-ABCA1 cells carry the wild-type (WT) ABCA1 insert, whereas BHK-A937V cells carry an alanine to valine mutant within the first nucleotide-binding domain of the transporter (34). Login to comment 116 ABCA1 p.Ala937Val ABCA1 p.Ala937Val RESULTS To determine whether functional ABCA1 influences extracellular ATP levels, we analyzed ATP concentrations in the medium of BHK-ABCA1 cells and compared them with the medium collected from Mock-BHK or BHK-A937V cells. Login to comment 118 ABCA1 p.Ala937Val Specifically, BHK-ABCA1 cells carry the wild-type (WT) ABCA1 insert, whereas BHK-A937V cells carry an alanine to valine mutant within the first nucleotide-binding domain of the transporter (34). Login to comment 120 ABCA1 p.Ala937Val Once induced with mifepristone overnight, BHK-ABCA1 and BHK-A937V cells express ABCA1 or ABCA1A937V , respectively, at comparable levels but not BHK-Mock cells (Fig. 1A, right 3 lanes). Login to comment 140 ABCA1 p.Ala937Val Most interestingly, mifepristone does not alter the extracellular ATP concentration in BHK-A937V cells (Fig. 1C), although ABCA1A937V is similarly expressed as WT ABCA1. Login to comment 145 ABCA1 p.Ala937Val Most interestingly, mifepristone does not alter the extracellular ATP concentration in BHK-A937V cells (Fig. 1C), although ABCA1A937V is similarly expressed as WT ABCA1. Login to comment