ABCMdb

PMID: 21628448

Watson MJ, Worthington EN, Clunes LA, Rasmussen JE, Jones L, Tarran R
Defective adenosine-stimulated cAMP production in cystic fibrosis airway epithelia: a novel role for CFTR in cell signaling.
FASEB J. 2011 Sep;25(9):2996-3003. Epub 2011 May 31., [PubMed]

Sentences

No. Mutations Sentence Comment

117 ABCC7 p.Asp572Asn To better understand how CFTR influences A2BR-stimulated cAMP production, we compared the effects of functional wild-type CFTR on cAMP production vs. no CFTR, CFTR mutants that never leave the endoplasmic reticulum (D572N; ref. 32), or CFTR mutants that are misprocessed and dysfunctional (⌬F508; ref. 33). Login to comment 118 ABCC7 p.Asp572Asn ABCC7 p.Asp572Asn To better understand how CFTR influences A2BR-stimulated cAMP production, we compared the effects of functional wild-type CFTR on cAMP production vs. no CFTR, CFTR mutants that never leave the endoplasmic reticulum (D572N; ref. 32), or CFTR mutants that are misprocessed and dysfunctional (⌬F508; ref. 33). Login to comment 119 ABCC7 p.Asp572Asn ADO-stimulated cAMP production was reduced with D572N CFTR to similar level as to CFTR-null cells (Fig. 6). Login to comment 166 ABCC7 p.Asp572Asn cAMP production was measured by ELISA in BHK cells and those stably transfected with either CFTR (BHKCFTR ) or mutant CFTRs (D572N and ⌬F508). Login to comment 167 ABCC7 p.Asp572Asn cAMP production was measured by ELISA in BHK cells and those stably transfected with either CFTR (BHKCFTR ) or mutant CFTRs (D572N and ⌬F508). Login to comment 192 ABCC7 p.Asp572Asn Expression of D572N CFTR, which does not escape the endoplasmic reticulum (32), did not potentiate ADO-stimulated cAMP production, suggesting that CFTR must be in the plasma membrane to regulate A2BR function (Fig. 6). Login to comment 193 ABCC7 p.Asp572Asn Expression of D572N CFTR, which does not escape the endoplasmic reticulum (32), did not potentiate ADO-stimulated cAMP production, suggesting that CFTR must be in the plasma membrane to regulate A2BR function (Fig. 6). Login to comment