PMID: 21628448

Watson MJ, Worthington EN, Clunes LA, Rasmussen JE, Jones L, Tarran R
Defective adenosine-stimulated cAMP production in cystic fibrosis airway epithelia: a novel role for CFTR in cell signaling.
FASEB J. 2011 Sep;25(9):2996-3003. Epub 2011 May 31., [PubMed]
Sentences
No. Mutations Sentence Comment
117 ABCC7 p.Asp572Asn
X
ABCC7 p.Asp572Asn 21628448:117:216
status: NEW
view ABCC7 p.Asp572Asn details
To better understand how CFTR influences A2BR-stimulated cAMP production, we compared the effects of functional wild-type CFTR on cAMP production vs. no CFTR, CFTR mutants that never leave the endoplasmic reticulum (D572N; ref. 32), or CFTR mutants that are misprocessed and dysfunctional (⌬F508; ref. 33). Login to comment
118 ABCC7 p.Asp572Asn
X
ABCC7 p.Asp572Asn 21628448:118:48
status: NEW
view ABCC7 p.Asp572Asn details
ABCC7 p.Asp572Asn
X
ABCC7 p.Asp572Asn 21628448:118:216
status: NEW
view ABCC7 p.Asp572Asn details
To better understand how CFTR influences A2BR-stimulated cAMP production, we compared the effects of functional wild-type CFTR on cAMP production vs. no CFTR, CFTR mutants that never leave the endoplasmic reticulum (D572N; ref. 32), or CFTR mutants that are misprocessed and dysfunctional (⌬F508; ref. 33). Login to comment
119 ABCC7 p.Asp572Asn
X
ABCC7 p.Asp572Asn 21628448:119:48
status: NEW
view ABCC7 p.Asp572Asn details
ADO-stimulated cAMP production was reduced with D572N CFTR to similar level as to CFTR-null cells (Fig. 6). Login to comment
166 ABCC7 p.Asp572Asn
X
ABCC7 p.Asp572Asn 21628448:166:125
status: NEW
view ABCC7 p.Asp572Asn details
cAMP production was measured by ELISA in BHK cells and those stably transfected with either CFTR (BHKCFTR ) or mutant CFTRs (D572N and ⌬F508). Login to comment
167 ABCC7 p.Asp572Asn
X
ABCC7 p.Asp572Asn 21628448:167:125
status: NEW
view ABCC7 p.Asp572Asn details
cAMP production was measured by ELISA in BHK cells and those stably transfected with either CFTR (BHKCFTR ) or mutant CFTRs (D572N and ⌬F508). Login to comment
192 ABCC7 p.Asp572Asn
X
ABCC7 p.Asp572Asn 21628448:192:14
status: NEW
view ABCC7 p.Asp572Asn details
Expression of D572N CFTR, which does not escape the endoplasmic reticulum (32), did not potentiate ADO-stimulated cAMP production, suggesting that CFTR must be in the plasma membrane to regulate A2BR function (Fig. 6). Login to comment
193 ABCC7 p.Asp572Asn
X
ABCC7 p.Asp572Asn 21628448:193:14
status: NEW
view ABCC7 p.Asp572Asn details
Expression of D572N CFTR, which does not escape the endoplasmic reticulum (32), did not potentiate ADO-stimulated cAMP production, suggesting that CFTR must be in the plasma membrane to regulate A2BR function (Fig. 6). Login to comment