ABCMdb

PMID: 21594796

Serohijos AW, Thibodeau PH, Dokholyan NV
Molecular modeling tools and approaches for CFTR and cystic fibrosis.
Methods Mol Biol. 2011;741:347-63., [PubMed]

Sentences

No. Mutations Sentence Comment

112 ABCC7 p.Phe508Ala We used the following structures for wild type and mutant NBD1s: wild type (PDB ID: 2BBO), F508del (PDB ID: 1XMJ), and F508A (PDB ID: 1XMI) (see Note 2). Login to comment 113 ABCC7 p.Phe508Ala The F508A mutant has been shown to exhibit intermediate folding defects compared to F508del (7), and thus is an interesting control for the folding simulations. Login to comment 124 ABCC7 p.Phe508Ala Simulation Protocol Using the simplified models described above, in our previous studies, we performed folding simulations for each NBD1-WT, NBD1-F508del, and NBD1-F508A. Login to comment 135 ABCC7 p.Phe508Ala (c) Contacts in NBD1-WT that perturbed in the F508A and F508del mutants. Login to comment 150 ABCC7 p.Phe508Ala We found that at the given tmax, wild type exhibited higher folding probability than the F508del, and F508A folding probability is intermediate to that of wild type and F508del. Login to comment