ABCMdb

PMID: 21594775

Pedemonte N, Zegarra-Moran O, Galietta LJ
High-throughput screening of libraries of compounds to identify CFTR modulators.
Methods Mol Biol. 2011;741:13-21., [PubMed]

Sentences

No. Mutations Sentence Comment

8 ABCC7 p.Gly551Asp ABCC7 p.Gly1349Asp In cystic fibrosis (CF), mutations affecting the CFTR gene cause a large variety of defects including altered CFTR channel gating (class III mutations such as G551D and G1349D) or impaired CFTR protein maturation (class II mutations such as F508del). Login to comment 39 ABCC7 p.Gly551Asp (a) Potentiator assay: FRT cells expressing G551D-CFTR. Login to comment 135 ABCC7 p.Gly551Asp ABCC7 p.Gly1349Asp ABCC7 p.Gly1349Asp 3.5.3. Conditions for the Screening of Potentiators on G551Dand G1349D-CFTR Cells The conditions are identical to those used to test potentiators on F508del-CFTR cells except that the step of incubation at low temperature is omitted. Login to comment 136 ABCC7 p.Gly551Asp Furthermore, higher concentrations of potentiators are required for G551D cells since this mutant is more refractory to pharmacological stimulation (e.g., genistein needs to be tested at 100-200 μM instead of 50 μM). Login to comment