ABCMdb

PMID: 21554545

Nagao K, Tomioka M, Ueda K
Function and regulation of ABCA1--membrane meso-domain organization and reorganization.
FEBS J. 2011 Sep;278(18):3190-203. doi: 10.1111/j.1742-4658.2011.08170.x. Epub 2011 Jun 13., [PubMed]

Sentences

No. Mutations Sentence Comment

80 ABCA1 p.Cys1477Arg This group is represented by the C1477R mutant of the second ECD (Table 2). Login to comment 81 ABCA1 p.Cys1477Arg Although C1477R mutant is expressed in the plasma membrane like wild-type ABCA1, apoA-I binding is abolished [9,10,49,50]. Login to comment 82 ABCA1 p.Trp590Ser The third group is the 'lipid translocation defect mutant`, represented by the W590S mutation in the first ECD of ABCA1 (Table 2). Login to comment 83 ABCA1 p.Trp590Ser The subcellular distribution of W590S is indistinguishable from that of wild-type ABCA1. Login to comment 84 ABCA1 p.Trp590Ser ABCA1 p.Trp590Ser Furthermore, W590S mutation does not affect apoA-I binding [9,10,49,50]; however, W590S mutation reduced phosphatidylserine (PS) flopping activity of ABCA1, detected with the annexin V binding assay [49], and impaired sodium taurocholate-dependent cholesterol and phospholipid efflux by ABCA1 [51]. Login to comment 85 ABCA1 p.Trp590Ser These results suggest that W590S mutation affects the lipid translocation activity of ABCA1 and that the two activities of ABCA1 (apoA-I binding and lipid translocation) can be separable (Fig. 3A). Login to comment 86 ABCA1 p.Trp590Ser Additionally, W590S mutation retarded the dissociation of apoA-I from ABCA1 [51]. Login to comment