ABCMdb

PMID: 21233638

Depatureaux A, Charpentier C, Leoz M, Unal G, Damond F, Kfutwah A, Vessiere A, Simon F, Plantier JC
Impact of HIV-1 group O genetic diversity on genotypic resistance interpretation by algorithms designed for HIV-1 group M.
J Acquir Immune Defic Syndr. 2011 Feb;56(2):139-45., [PubMed]

Sentences

No. Mutations Sentence Comment

34 ABCC8 p.Leu210Tyr ABCC8 p.Val118Cys At positions associated with resistance to NRTI, all HIV-O sequenced samples (67 of 67, 100%) harbored the uncommon mutations V118C and L210Y, and 3% (2 of 67) exhibited the T69N mutation (Fig. 1A). Login to comment 49 ABCC8 p.Ile93Leu The following minor resistance mutations, defined by at least 1 of the 3 algorithms, were found in more than 75% of the sequences: L10V (75%), I15V (91%), E35N (97%), M36I (99%), K43T (75%), Q58E (97%), I62V (100%), I64V (84%), H69R (88%), A71V (94%), L89I (88%), and I93L (100%) (Fig. 1C). Login to comment 104 ABCC8 p.Leu210Tyr ABCC8 p.Val118Cys DISCUSSION We report here a wide range of natural polymorphism in the RT and protease regions in a large series of HIV-O samples issued from 68 ARV-naive patients, extending similar findings previously reported for smaller series.5,11-13,21 The uncommon mutations, V118C and L210Y, were found naturally present in 100% of the RT sequences. Login to comment 106 ABCC8 p.Leu210Tyr ABCC8 p.Val118Cys The phenotypic characteristics of the V118C and L210Y mutants are currently unknown and should be determined to assess their impact on viral susceptibility. Login to comment 113 ABCC8 p.Ile93Leu Furthermore, HIV-OANT70 and HIV-2ROD reference sequences shared 6 resistance-associated mutations (M36I, Q58E, I62V, A71V, L89I, I93L) and 1 uncommon mutation (I13A).25-27 Thus, as for HIV-2,27-29 HIV-O natural polymorphism is likely to have consequences for susceptibility to the different PI. Login to comment