PMID: 21179202

Nardinocchi L, Pantisano V, Puca R, Porru M, Aiello A, Grasselli A, Leonetti C, Safran M, Rechavi G, Givol D, Farsetti A, D'Orazi G
Zinc downregulates HIF-1alpha and inhibits its activity in tumor cells in vitro and in vivo.
PLoS One. 2010 Dec 13;5(12):e15048., [PubMed]
Sentences
No. Mutations Sentence Comment
184 ABCB1 p.Pro402Ala
X
ABCB1 p.Pro402Ala 21179202:184:784
status: NEW
view ABCB1 p.Pro402Ala details
 The plasmid reporter used were the VEGF-luc vector that contains the hypoxia response elements (HREs) of VEGF gene promoter cloned upstream of a luciferase transcriptional reporter and the VEGF-luc reporter mutated in the HRE sites [24] (kindly provided by C. Gaetano, IDI, IRCCS, Rome, Italy), the ODD-luc plasmid that encodes fusion proteins consisting of HIF-1a (aa 530-652, a unique oxygen-dependent degradation domain (ODD) fused to the N terminus of firefly luciferase [25]; the expression vectors encoding the dominant negative form of HIF-1a without DNA binding domain and transactivation domain (pCEP4-HIF-1aDN) [23] (kindly provided by B.H. Jiang, Nanjing Medical University, China), the Flag-tagged HIF-1a expression vector and the Flag-tagged HIF-1a with prolyl mutations P402A and P564A [26] (kindly provided by G.L. Semenza, The Johns Hopkins University School of Medicine, Baltimore, MD, USA) were also used. Login to comment