ABCMdb

PMID: 21150129

Yamamoto YH, Kimura T, Momohara S, Takeuchi M, Tani T, Kimata Y, Kadokura H, Kohno K
A novel ER J-protein DNAJB12 accelerates ER-associated degradation of membrane proteins including CFTR.
Cell Struct Funct. 2010;35(2):107-16. Epub 2010 Dec 8., [PubMed]

Sentences

No. Mutations Sentence Comment

86 ABCC7 p.His139Gln Since the highly conserved HPD motif in J-domains is generally required for their interaction with the partner Hsp70 family chaperones (Kampinga and Craig, 2010), we constructed a DNAJB12 mutant carrying a substitution mutation that alters this HPD motif to QPD (H139Q). Login to comment 87 ABCC7 p.His139Gln When the H139Q mutant version of DNAJB12- Flag was expressed in HeLa cells, its expression level was almost comparable to that of wild-type DNAJB12-Flag (compare lane 3 with lane 2 in the bottom panel). Login to comment 106 ABCC7 p.His139Gln ABCC7 p.His139Gln On the other hand, the coexpression of the H139Q mutant version of DNAJB12-Flag gave a more modest effect, as only the level of the C form was decreased significantly (lane 3) (see Discussion section for the interpretation of this result). Login to comment 107 ABCC7 p.His139Gln As shown in the lower panel of Fig. 3B, GFP-WT-CFTR was co-immunoprecipitated efficiently with the wild-type DNAJB12 but not with the H139Q mutant, indicating that the HPD motif in the J-domain of DNAJB12 is important for the efficient binding Fig. 2. Login to comment 117 ABCC7 p.His139Gln (A) HeLa cells were mock-transfected or transfected with a plasmid to express DNAJB12-Flag or DNAJB12 H139Q-Flag. Login to comment 183 ABCC7 p.His139Gln This association was abolished by a mutation (H139Q) in the J-domain of DNAJB12 (Fig. 3A, B). Login to comment