ABCMdb

PMID: 20624916

Morino M, Natsui S, Ono T, Swartz TH, Krulwich TA, Ito M
Single site mutations in the hetero-oligomeric Mrp antiporter from alkaliphilic Bacillus pseudofirmus OF4 that affect Na+/H+ antiport activity, sodium exclusion, individual Mrp protein levels, or Mrp complex formation.
J Biol Chem. 2010 Oct 1;285(40):30942-50. Epub 2010 Jul 12., [PubMed]

Sentences

No. Mutations Sentence Comment

176 ABCC1 p.Gly392Arg MrpA-G392R and MrpE-T113Y were also included in Group 3 because of their very low antiport level. Login to comment 180 ABCC1 p.Gly392Arg MrpC-G82I and MrpA-G392R (Group 3) were the sites of the identified original mutations, which led to the non-alkaliphilic phenotype in B. halodurans C-125. Login to comment 181 ABCC1 p.Gly392Arg MrpA-G392R and MrpE-T113Y were also included in Group 3 because of their very low antiport level. Login to comment 185 ABCC1 p.Gly392Arg ABCC1 p.Gly392Ala MrpC-G82I and MrpA-G392R (Group 3) were the sites of the identified original mutations, which led to the non-alkaliphilic phenotype in B. halodurans C-125. Login to comment 190 ABCC1 p.Gly392Ala Neither of the two glycines that were mutated in the original B. halodurans C-125 mutagenesis is crucial for antiport inasmuch as the MrpA-G392A and MrpC-G82P mutants of B. pseudofirmus OF4 yielded mutant Mrp systems with no functional phenotype of significance and only a partial reduction in the membrane levels of MrpC itself for the MrpC-G82P mutant (see Group 8). Login to comment 200 ABCC1 p.Thr75Ala Group 6 contains two mutants with single mutations in MrpB and MrpC, MrpB-F41A and MrpC-T75A. Login to comment 205 ABCC1 p.Thr75Ala Group 6 contains two mutants with single mutations in MrpB and MrpC, MrpB-F41A and MrpC-T75A. Login to comment 215 ABCC1 p.Gly392Ala Group 8 contains the remaining mutations: MrpA-Y136A, MrpA-H230A, MrpA-W232A, MrpA-Y258A, MrpA-H345A, MrpA-G392A, MrpA-F405A, MrpB-H34A, MrpC-G82P, MrpD-F135A, MrpD-F136A, MrpD-F136E, MrpD-F136T, MrpD-W228A, MrpE-P114A, and MrpF-P28G. Login to comment 220 ABCC1 p.Gly392Ala Group 8 contains the remaining mutations: MrpA-Y136A, MrpA-H230A, MrpA-W232A, MrpA- Y258A, MrpA-H345A, MrpA-G392A, MrpA-F405A, MrpB-H34A, MrpC-G82P, MrpD-F135A, MrpD-F136A, MrpD-F136E, MrpD-F136T, MrpD- W228A, MrpE-P114A, and MrpF- P28G. Login to comment