ABCMdb

PMID: 20008117

Cholon DM, O'Neal WK, Randell SH, Riordan JR, Gentzsch M
Modulation of endocytic trafficking and apical stability of CFTR in primary human airway epithelial cultures.
Am J Physiol Lung Cell Mol Physiol. 2010 Mar;298(3):L304-14. Epub 2009 Dec 11., [PubMed]

Sentences

No. Mutations Sentence Comment

266 ABCC7 p.Asn900Asp ABCC7 p.Asn894Asp A: Western blot of lysates of HAE cells adenovirally expressing CFTR N894D N900D, in which asparagine residues N894 and N900 are replaced with aspartate, confirmed lack of glycosylation. Login to comment 267 ABCC7 p.Asn900Asp ABCC7 p.Asn894Asp B: primary HAE cultures were adenovirally transduced to express wild-type CFTR and a nonglycosylated variant (N894D N900D) of CFTR. Login to comment 268 ABCC7 p.Asn900Asp ABCC7 p.Asn894Asp A: Western blot of lysates of HAE cells adenovirally expressing CFTR N894D N900D, in which asparagine residues N894 and N900 are replaced with aspartate, confirmed lack of glycosylation. Login to comment 269 ABCC7 p.Asn900Asp ABCC7 p.Asn894Asp B: primary HAE cultures were adenovirally transduced to express wild-type CFTR and a nonglycosylated variant (N894D N900D) of CFTR. Login to comment 270 ABCC7 p.Asn900Asp ABCC7 p.Asn894Asp Time course experiments were performed on 3 different HAE cultures; 1 representative example is shown. D: immunofluorescence labeling of sodium-potassium-chloride cotransporter isoform 1 (NKCC1) confirms that internalized CFTR N894D N900D is transported to basolateral compartments. Login to comment 271 ABCC7 p.Asn900Asp ABCC7 p.Asn894Asp HAE cultures were adenovirally transduced to express CFTR N894D N900D. Login to comment 272 ABCC7 p.Asn900Asp ABCC7 p.Asn894Asp Time course experiments were performed on 3 different HAE cultures; 1 representative example is shown. D: immunofluorescence labeling of sodium-potassium-chloride cotransporter isoform 1 (NKCC1) confirms that internalized CFTR N894D N900D is transported to basolateral compartments. Login to comment 273 ABCC7 p.Asn900Asp ABCC7 p.Asn894Asp HAE cultures were adenovirally transduced to express CFTR N894D N900D. Login to comment