ABCMdb

PMID: 20004175

de Foresta B, Vincent M, Gallay J, Garrigos M
Interaction with membrane mimics of transmembrane fragments 16 and 17 from the human multidrug resistance ABC transporter 1 (hMRP1/ABCC1) and two of their tryptophan variants.
Biochim Biophys Acta. 2010 Mar;1798(3):401-14. Epub 2009 Dec 11., [PubMed]

Sentences

No. Mutations Sentence Comment

63 ABCC1 p.Cys1209Ser ABCC1 p.Cys1205Ser For this study, we synthesized a Cys1205Ser and Cys1209Ser double mutant of TM16 (mTM16), containing a single-Trp residue, W1198, close to the N-terminus (or W4 in the peptide sequence) (Table 1). Login to comment 65 ABCC1 p.Phe1213Trp We synthesized a TM16 Trp variant (W19-mTM16), in which the aromatic residue Phe1213 was replaced by a tryptophan residue, to probe a part of TM16 closer to the C-terminus (i.e., to the extracellular side in the whole protein). Login to comment 67 ABCC1 p.Trp1246Tyr ABCC1 p.Ala1227Lys ABCC1 p.Tyr1236Trp Similarly, a Ala1227Lys mutant of the TM17 fragment (mTM17) containing Trp1246 (or W20) [37] and the Tyr1236Trp and Trp1246Tyr variant of mTM17 (W10-mTM17), with its Trp closer to the extracellular side in the whole protein, were also synthesized (Table 1). Login to comment 346 ABCC1 p.Trp1246Tyr ABCC1 p.Tyr1236Trp For TM17, the Trp variant results from the two point mutations: Y1236W and W1246Y, so that the overall effect on ΔGu (interfacial partitioning free energy of the peptide) is null. Login to comment 348 ABCC1 p.Trp1198Tyr For TM16, the variant results from the following two point mutations: W1198Y and F1113W. Login to comment