ABCMdb

PMID: 19880661

Ierano T, Cescutti P, Leone MR, Luciani A, Rizzo R, Raia V, Lanzetta R, Parrilli M, Maiuri L, Silipo A, Molinaro A
The lipid A of Burkholderia multivorans C1576 smooth-type lipopolysaccharide and its pro-inflammatory activity in a cystic fibrosis airways model.
Innate Immun. 2010 Dec;16(6):354-65. Epub 2009 Oct 30., [PubMed]

Sentences

No. Mutations Sentence Comment

4 ABCC7 p.Trp1282* The structure of B. multivorans lipid A was attained by chemical, mass spectrometry and nuclear magnetic resonance analyses whereas its biological activity was assessed on the intestinal epithelial cell line CACO-2 cells, on the airway epithelial IB3-1 cells, carrying the ÁF508/W1282X CFTR mutation and on an ex vivo model of culture explants of nasal polyps. Login to comment 15 ABCC7 p.Trp1282* The structure of B. multivorans lipid A was attained by chemical, mass spectrometry (MS) and nuclear magnetic resonance (NMR) analyses whereas its biological activity was assessed on the intestinal epithelial cell line CACO-2 cells, on the IB3-1 cells, carrying the ÁF508/W1282X CFTR mutation and on an ex vivo model of culture explants of nasal polyps. Login to comment 43 ABCC7 p.Trp1282* Patients and ex vivo cultures of nasal polyp mucosal biopsies Five consecutive cystic fibrosis patients carrying the common CFTR mutations (F508del/F508del, F508del/W1282X) with idiopathic polyposis underwent surgical treatment. Login to comment 47 ABCC7 p.Trp1282* Cell culture Human cystic fibrosis bronchial epithelial cell line IB3-1, carrying the F508del/W1282X CFTR mutation, or intestinal CACO-2 cell line (LGC Promochem, Milan, Italy) were cultured as recommended by the American Type Culture Collection (ATCC). Login to comment 129 ABCC7 p.Trp1282* Effects of B. multivorans lipid A on cystic fibrosis airways To investigate whether B. multivorans lipid A was effective in triggering an inflammatory response in cystic fibrosis airways, we used an epithelial cell line carrying a common ÁF508/W1282X CFTR mutation. Login to comment 130 ABCC7 p.Trp1282* Effects of B. multivorans lipid A on cystic fibrosis airways To investigate whether B. multivorans lipid A was effective in triggering an inflammatory response in cystic fibrosis airways, we used an epithelial cell line carrying a common ÁF508/W1282X CFTR mutation. Login to comment