ABCMdb

PMID: 19470507

Sharma M, Manoharlal R, Shukla S, Puri N, Prasad T, Ambudkar SV, Prasad R
Curcumin modulates efflux mediated by yeast ABC multidrug transporters and is synergistic with antifungals.
Antimicrob Agents Chemother. 2009 Aug;53(8):3256-65. Epub 2009 May 26., [PubMed]

Sentences

No. Mutations Sentence Comment

77 ABCC1 p.Gly1362Ala ABCC1 p.Gly1362Ala ABCC1 p.Ala1347Gly ABCC1 p.Ala1347Gly Strain name or mutation Genotype or descriptiona Source 1 AD1-8u- MAT␣ pdr1-3 his1 ura ⌬yor1::hisG ⌬snq2::hisG ⌬pdr5::hisG ⌬pdr10::hisG ⌬pdr11::hisG ⌬ycf1::hisG pdr3::hisG ⌬pdr15::hisG 7 2 AD-CDR1 AD1-8u- cells harboring the CaCDR1-GFP ORF integrated at the PDR5 locus 27 3 A1346G CDR1-GFP cells carrying an A1346G mutation in the CDR1 ORF and integrated at the PDR5 locus 24 4 A1347G CDR1-GFP cells carrying an A1347G mutation in the CDR1 ORF and integrated at the PDR5 locus 24 5 T1351A CDR1-GFP cells carrying a T1351A mutation in the CDR1 ORF and integrated at the PDR5 locus 24 6 F1360A CDR1-GFP cells carrying an F1360A mutation in the CDR1 ORF and integrated at the PDR5 locus 24 7 G1362A CDR1-GFP cells carrying a G1362A mutation in the CDR1 ORF and integrated at the PDR5 locus 24 8 L1358A CDR1-GFP cells carrying an L1358A mutation in the CDR1 ORF and integrated at the PDR5 locus 24 9 T1355A CDR1-GFP cells carrying a T1355A mutation in the CDR1 ORF and integrated at the PDR5 locus 24 10 G682A CDR1-GFP cells carrying a G682A mutation in the CDR1 ORF and integrated at the PDR5 locus Puri et al., unpublished data 11 AD-CaMDR1 AD1-8u- cells harboring the CaMDR1-GFP ORF integrated at the PDR5 locus 22 12 AD-CDR2 AD1-8u- cells harboring the CaCDR2-GFP ORF integrated at the PDR5 locus 17 13 AD-PDR5 AD1-8u- cells harboring the ScPDR5-GFP ORF integrated at the PDR5 locus 17 14 CAI4 ⌬ura3::imm434/⌬ura3::imm434 8 a ORF, open reading frame. Login to comment 172 ABCC1 p.Gly1362Ala ABCC1 p.Ala1347Gly Out of 21 residues of transmembrane segment 11, substitution of 5 residues, namely, A1346G, A1347G, T1351A, F1360A, and G1362A, abrogated the efflux of R6G, while the rest of the mutant variants of CaCdr1p showed unaltered efflux (Fig. 6A). Login to comment