ABCMdb

PMID: 18485890

Barakat S, Turcotte S, Demeule M, Lachambre MP, Regina A, Baggetto LG, Beliveau R
Regulation of brain endothelial cells migration and angiogenesis by P-glycoprotein/caveolin-1 interaction.
Biochem Biophys Res Commun. 2008 Aug 1;372(3):440-6. Epub 2008 May 15., [PubMed]

Sentences

No. Mutations Sentence Comment

52 ABCB1 p.Phe37Ala After 24 h, the cells were transiently transfected, in serum-free medium, with FuGENE 6 transfection reagent using 1 or 2 lg of DNA (either WT, YWAA or F37A). Login to comment 132 ABCB1 p.Phe37Ala In the other mutant, F37A, Phe37 (F) was replaced with Ala. Login to comment 141 ABCB1 p.Phe37Ala The caveolin-1/P-gp interaction was reduced by 25 and 35%, respectively, for the YWAA and F37A mutations (Fig. 3C). Login to comment 142 ABCB1 p.Phe37Ala Hoechst dye accumulation was decreased by similar amounts 22 and 28%, respectively, for the YWAA and F37A mutant transfected cells compared to the wild-type- transfected cells (Fig. 3D). Login to comment 161 ABCB1 p.Phe37Ala However, in the case of the mutants YWAA and F37A, the length of the capillary-like tubular structure was increased by about 75% compared to the wild-type MDR1 transfected cells. Login to comment 186 ABCB1 p.Phe37Ala However, the migration of cells transfected with either YWAA or F37A MDR1 was increased by 75% compared to the wild-type MDR1 transfected cells. Login to comment