ABCMdb

PMID: 18463677

Chapuy B, Koch R, Radunski U, Corsham S, Cheong N, Inagaki N, Ban N, Wenzel D, Reinhardt D, Zapf A, Schweyer S, Kosari F, Klapper W, Truemper L, Wulf GG
Intracellular ABC transporter A3 confers multidrug resistance in leukemia cells by lysosomal drug sequestration.
Leukemia. 2008 Aug;22(8):1576-86. Epub 2008 May 8., [PubMed]

Sentences

No. Mutations Sentence Comment

21 ABCA3 p.Asn568Asp The pEGFP-N1-ABCA3 wild-type (wt) and pEGFP-N1-ABCA3 N568D mutant plasmids were described previously.10 The monoclonal mouse antibody to early endosomal antigen 1 (EEA1) was obtained commercially (Transduction Laboratories), the antibodies to lysosomal-associated membrane protein 1 (LAMP1, code H4A3) and LAMP2 (code H4B4) were from the Developmental Studies Hybridoma Bank (Iowa City, IA, USA). Login to comment 62 ABCA3 p.Asn568Asp ABCA3 p.Asn568Asp Oldendorf, Germany) with 10 mg of either pmax-GFP, pEGFP- N1-ABCA3 wt or pEGFP-N1-ABCA3 N568D, pulsed in an EasyjecT (Equi Bio, Middlesex, UK) electroporator (300 V, 1050 mF, 99 O, t: 104 ms) and incubated for 3 h in 400 ml OptiMEM before further expansion with 1.5 ml of complete growth medium overnight. Login to comment 139 ABCA3 p.Asn568Asp A nonfunctional, mutated variant N568D-eGFP of ABCA3 does not induce drug resistance, and specific siRNA against ABCA3 increases the cytotoxic efficacy of DNR, etoposide and Ara-C (Supplementary Figure 2C, D). Login to comment