ABCMdb

PMID: 18385361

Zakeri S, Afsharpad M, Kazemzadeh T, Mehdizadeh K, Shabani A, Djadid ND
Association of pfcrt but not pfmdr1 alleles with chloroquine resistance in Iranian isolates of Plasmodium falciparum.
Am J Trop Med Hyg. 2008 Apr;78(4):633-40., [PubMed]

Sentences

No. Mutations Sentence Comment

60 ABCB1 p.Ala220Ser Following amplification of the fragments concerned, polymorphisms in the pfcrt and pfmdr1 genes were assessed by the mutation-specific restriction endonuclease digestion to detect single nucleotide polymorphisms (SNPs) in pfcrt at positions K76T, A220S, Q271E, N326S/D, I356T/L, and R371I/T and in pfmdr1 at positions N86Y/H, Y184F, S1034C, N1042D, and D1246Y.45,46 A number of restriction enzymes were used for RFLP of PCR products.45,46 For pfcrt, the PCR products were digested with ApoI, BglI, XmnI, MseI, CaiI, and AFlII to determine the polymorphisms at codons 76, 220, 271, 326, 356, and 371, respectively (detailed information is available on the internet at http://medschool.umaryland.edu/cvd/ nejm2001djimde.asp). Login to comment 88 ABCB1 p.Ala220Ser Sequence analyses of codons N326S/D TABLE 1 Distribution of pfcrt polymorphisms in pre-and post-treatment samples from Iran based on PCR/RFLP* Pfcrt K76T A220S Q271E N326S/D I356T/L R371I/T Total (%) Pre-treatment K A Q N I/L R 4 (2.3%) T A Q S/D I/L R 1 (0.6%) T S Q S/D I/L R 170 (97.1%) T = 97.7% S = 97% E = 0 S/D = 97.7% I/L = 100% I/T = 0 175 (100) Post-treatment K A Q N I/L R - T A Q S/D I/L R 1 (4%) T S Q S/D I/L R 24 (96%) T = 100% S = 96% E = 0 S/D = 100% I/L = 100% I/T = 0 25 (100%) * Analysis of codon N326S/D by MseI restriction enzyme digestion showed that 97.7% of our samples had either amino acid serine (S) or aspartic acid (D). Login to comment