ABCMdb

PMID: 18359958

Faulkner LE, Panagotopulos SE, Johnson JD, Woollett LA, Hui DY, Witting SR, Maiorano JN, Davidson WS
An analysis of the role of a retroendocytosis pathway in ABCA1-mediated cholesterol efflux from macrophages.
J Lipid Res. 2008 Jun;49(6):1322-32. Epub 2008 Mar 22., [PubMed]

Sentences

No. Mutations Sentence Comment

131 ABCA1 p.Val93Cys The V93C mutant was chosen for further studies because it exhibited the highest levels of expression in our recombinant system. Login to comment 132 ABCA1 p.Val93Cys ABCA1 p.Val93Cys 1324 Journal of Lipid Research Volume 49, 2008 atHealthScienceLibraryCB#7585,onSeptember24,2012www.jlr.orgDownloadedfrom 0.DC1.html Next, the ability to visualize fluorescent apoA-I (V93C) within RAW264.7 macrophages treated with cAMP to induce expression of ABCA1 was assessed. Login to comment 133 ABCA1 p.Val93Cys Panels 1 and 2 of Fig. 2A show an optical slice taken through the center of the macrophage by confocal microscopy after incubation with fluorescent apoA-I (V93C) for 2 h. Login to comment 143 ABCA1 p.Val93Cys To evaluate this possibility, the effect of cAMP treatment on the uptake of dextran polymers labeled with the identical Alexa Fluor 546 probe as that present on our apoA-I (V93C) was studied. Login to comment 181 ABCA1 p.Val93Cys Colocalization of Alexa Fluor apoA-I (V93C) with LysoTracker Green in RAW cells. Login to comment 190 ABCA1 p.Val93Cys Then, either 10 mg/ml Alexa Fluor-labeled apoA-I (V93C) 6 400 mg/ml unlabeled apoA-I competitor, or 18 mg/ml Alexa Fluor-labeled dextran beads were added 6 0.3 mM cAMP for 2 h. Login to comment 223 ABCA1 p.Val93Cys Pulse-chase incubation of Alexa Fluor apoA-I (V93C) in RAW cells. Login to comment 224 ABCA1 p.Val93Cys Pulse-chase incubation of Alexa Fluor apoA-I (V93C) in RAW cells. Login to comment