ABCMdb

PMID: 18328253

Loo TW, Clarke DM
Mutational analysis of ABC proteins.
Arch Biochem Biophys. 2008 Aug 1;476(1):51-64. Epub 2008 Mar 5., [PubMed]

Sentences

No. Mutations Sentence Comment

127 ABCG2 p.Asp210Asn In another approach to determine if BCRP functioned as a dimer, inactive mutants were constructed by introducing a D210N change in the Walker B motif [44]. Login to comment 128 ABCG2 p.Asp210Asn Chimeric BCRP molecules were constructed that contained two wild-type BCRP molecules fused together or a wild-type molecule fused to a D210N mutant. Login to comment 130 ABCG2 p.Asp210Asn Expression of the chimeric molecule containing a D210N mutation, however, resulted in a dominant-negative phenotype, as the protein was expressed at the cell surface but was not functional. Login to comment 313 ABCB1 p.Gly341Cys ABCB3 p.Val331Cys Seven TM6 cysteine mutants (V331C, T333C, F335C, S337C, L339C, G341C, F343C) were labeled in the presence or absence of AMPPNP or after vanadate trapping of nucleotide. Login to comment 315 ABCB1 p.Gly341Cys ABCC5 p.Phe343Cys ABCC5 p.Leu339Cys ABCC5 p.Phe335Cys ABCC5 p.Ser337Cys ABCB3 p.Val331Cys Seven TM6 cysteine mutants (V331C, T333C, F335C, S337C, L339C, G341C, F343C) were labeled in the presence or absence of AMPÁPNP or after vanadate trapping of nucleotide. Login to comment 317 ABCC5 p.Phe343Cys For example, only mutant F343C was significantly labeled with fluorescein maleimide in the absence of nucleotide and labeling was lost in the presence of AMPÁPNP or after vanadate trapping of nucleotide. Login to comment 318 ABCB3 p.Val331Cys For example, mutants V331C and L339C could be labeled in the absence of nucleotide or after vanadate trapping but not in the presence of AMPPNP. Login to comment 320 ABCC5 p.Leu339Cys ABCB3 p.Val331Cys For example, mutants V331C and L339C could be labeled in the absence of nucleotide or after vanadate trapping but not in the presence of AMPÁPNP. Login to comment 321 ABCC5 p.Ser337Cys By contrast, labeling of mutant S337C only occurred after vanadate trapping of nucleotide. Login to comment 368 ABCB1 p.Ile306Arg In drug-stimulated ATPase assays, it was found that the I306R mutation completely abolished vinblastine-stimulated ATPase activity but had little effect on rhodamine-stimulated ATPase activity [112]. Login to comment 370 ABCB1 p.Ile306Arg In drug-stimulated ATPase assays, it was found that the I306R mutation completely abolished vinblastine-stimulated ATPase activity but had little effect on rhodamine-stimulated ATPase activity [112]. Login to comment 372 ABCB1 p.Ile306Arg The effects of I306R mutation on vinblastine interactions were similar in the ATPase and cross-linking assays. Login to comment 373 ABCC1 p.Phe728Cys ABCC5 p.Leu339Cys Introduction of the I306R(TM5) mutation into mutant L339C/F728C reduced its apparent affinity for vinblastine more than 60-fold but had little effect on its apparent affinity for rhodamine. Login to comment 374 ABCB1 p.Ile306Arg ABCB1 p.Ile306Arg ABCB1 p.Ile306Arg The effects of I306R mutation on vinblastine interactions were similar in the ATPase and cross-linking assays. Login to comment 376 ABCB1 p.Ile306Arg ABCB1 p.Ile306Arg ABCC5 p.Phe343Arg ABCC5 p.Phe343Arg For example, the L65R, T199R and I306R mutations inhibited vinblastine interactions, the I306R and F343R changes inhibited binding of cyclosporin A and the F343R mutation inhibited binding of rhodamine. Login to comment