ABCMdb

PMID: 18095031

Huang MY, Wang YH, Chen FM, Lee SC, Fang WY, Cheng TL, Hou MF, Wang JY, Lin SR
Multiple Genetic Polymorphisms of GSTP1 313AG, MDR1 3435CC, and MTHFR 677CC highly correlated with early relapse of breast cancer patients in Taiwan.
Ann Surg Oncol. 2008 Mar;15(3):872-80. Epub 2007 Dec 19., [PubMed]

Sentences

No. Mutations Sentence Comment

4 ABCB1 p.Ala313Gly Results: The results showed that the genotype distribution of GSTP1 A313G, MTHFR C677T, and TS 3R3R in Taiwanese subjects differed significantly from the distribution in Caucasians. Login to comment 36 ABCB1 p.Ala313Gly GSTP1 exon5 A313G, MDR1 C3435T, and MDR1 C3435T were restricted by BsmAI, DpnII, and HinfI, respectively. Login to comment 54 ABCB1 p.Ala313Gly PCR-RFLP Analysis Results of Chemotherapeutic Agent-Related Genetic Polymorphisms In our study, chemotherapeutic agent-related genetic polymorphisms are GSTP1 A313G, MDR1 C3435T, MTHFR C667T, and TS double or triple tandem repeat. Login to comment 60 ABCB1 p.Ala313Gly Comparison of Genotypes Distribution between Taiwanese Breast Cancer Patients and Other Races We compared the distribution of genetic polymorphisms, such as GSTP1 A313G (AA, AG, GG), MDR1 C3435T (CC, CT, TT), MTHFR C677T (CC, CT, TT), and TS tandem repeat (3R3R, 2R3R, 2R2R), in various races, including Caucasian, Korean, African American, Chinese, Japanese American, and Taiwanese subjects from our work and literature reviews. Login to comment 62 ABCB1 p.Ala313Gly The distribution of GSTP1 A313G genotypes in our data show significant differences from that of Caucasian (P = .001) and African American populations (P = .001)13 but no significant differences from Chinese (P = .410)14 and Korean populations (P = .242).15 The distribution of MDR1 C3435T genotypes also revealed significant differences from the Caucasian population (P = .003).16 Significantly different distributions of MTHFR C677T (CC, CT, TT) genotypes are evident when comparing our data with Caucasian (P = .001),17 Chinese (P < .0001),18 Korean (P < .0001),19 African American (P < .0001),20 and Japanese American populations (P < .0001),21 but our study is consistent with the other Taiwanese report (P = .150).22 Our study and the another Taiwanese study22 both showed that MTHFR C677T CC appeared more frequently than CT and TT, while another racial study showed that MTHFR C677T CT was the most frequent genetic polymorphism. Login to comment 64 ABCB1 p.Ala313Gly Characteristics of the studied polymorphisms with primer sequences and restriction enzymes Gene polymorphism Primers PCR size PCR condition Restriction enzyme GSTP1Ile105Val (A313G) [F]:5'-GTAGTTTGCCCAAGGTCAAG-3' 433 Annealing: 62°C, 20 sec BsmAI [R]:5'-AGCCACCTGAGGGGTAAG-3' MDR1Ile1145Ile (C3435T) [F]:5'-gatctgtgaactcttgttttc-3' 244 Annealing: 58°C, 20 sec DpnII [R]:5'-GAAGAGAGACTTACATTAGGC-3' MTHFRAla222Val (C677T) [F]:5'-TGGCAGGTTACCCCAAAGGC-3' 400 Annealing: 70°C, 20 sec HinfI [R]:5'-ACTGTTGCTGGGTTTTGGGG-3' TS 28 bp tandem repeat [F]:5'-GTGGCTCCTGCGTTTCCCCC-3' 240 Annealing: 72°C, 15 sec - [R]:5'-TCCGAGCCGGCCACAGGCAT-3' patients presented TS 2R3R as the most frequent genetic polymorphism. Login to comment 68 ABCB1 p.Ala313Gly Correlation between Early Relapse and Genotypes of Chemotherapeutic Agent-Related Genes The correlation between genetic polymorphisms (MTHFR C677T, MDR1 C3435T, GSTP1 A313G, and TS double or triple tandem repeat) and patients with or without early relapse was analyzed. Login to comment 78 ABCB1 p.Ala313Gly GSTP1 exon5 A313G was restricted by BsmAI and resulted in: 329bp and 104bp GSTP1 313AA fragments; 329bp, 222bp, 107bp, and 104bp GSTP1 313AG fragments; and 222bp, 104bp, and 107bp GSTP1 313GG fragments. Login to comment 107 ABCB1 p.Ala313Gly (%) GSTP1 A313G Sweeney13 Kim15 Sweeney13 Egan14 AA 93 (48.4) 122 (71.3) 17 (35) 723 (63.5) - - 122 (64.2) AG 80 (41.7) 44 (25.7) 27 (56) 363 (31.9) - - 65 (33.9) GG 19 (9.9) 5 (2.9) 4 (8) 53 (4.6) - - 5 (1.9) MDR1 C3435T Kafka16 CC 21 (21) - - - - - 79 (41.1) CT 57 (57) - - - - - 79 (41.1) TT 22 (22) - - - - - 34 (17.8) MTHFR C677T Campbell17 Lee19 Martin20 Shrubsole18 Chou22 Le Marchand21 CC 140 (41.8) 58 (31.2) 114 (81) 355 (34.2) 73 (51.4) 135 (42.5) 112 (58.3) CT 162 (48.4) 96 (51.6) 27 (19) 507 (48.8) 51 (35.9) 140 (44.0) 67 (34.9) TT 33 (9.8) 32 (17.2) 0 (0) 176 (17.0) 18 (12.7) 43 (13.5) 13 (6.8) TS tandem repeat Largillier24 Zhai23 3R3R 27 (32.7) - - 279 (64.6) - - 129 (67.2) 2R3R 43 (50) - - 130 (30.1) - - 61 (31.8) 2R2R 13 (17.3) - - 23 (5.3) - - 2 (1) a authorn in this table are presented with reference number (n ) and the abbreviated name of the first author in the previously reported paper. Login to comment 120 ABCB1 p.Ala313Gly After comparing the Taiwanese with other races with regard to genetic polymorphism, we found that the Taiwanese, Korean, and Chinese demonstrate a higher frequency of GSTP1 A313G AA than Caucasians and African Americans do. Taiwanese demonstrate a higher frequency of MTHFR C677T CC than other races do. Caucasians demonstrate a higher frequency of MDR1 C3435T CT and TS tandem repeat 2R than the Taiwanese do. The differences in genetic polymorphisms may explain the variety of chemotherapeutic agent-related responses that occur worldwide. Login to comment