ABCMdb

PMID: 18035039

Lawson J, O'Mara ML, Kerr ID
Structure-based interpretation of the mutagenesis database for the nucleotide binding domains of P-glycoprotein.
Biochim Biophys Acta. 2008 Feb;1778(2):376-91. Epub 2007 Nov 1., [PubMed]

Sentences

No. Mutations Sentence Comment

674 ABCB1 p.Gly427Cys Two studies have proposed that the Walker-A cysteine residues can be cross-linked to the opposite Walker-A motif [73,74], either through a direct disulphide between the two naturally occurring Walker-A cysteines [74] or between the Walker-A cysteine of NBD2 and a G427C Walker-A NBD1 mutation [73]. Login to comment 676 ABCB1 p.Leu1176Cys More recently, in the light of the NBD sandwich dimer, Loo et al. have demonstrated that the Walker-A cysteine can be cross-linked to a cysteine residue introduced within the opposite signature motif (L1176C, [19]), 10 &#c5; apart (Cb1;-Cb1;, Table S1). Login to comment 679 ABCB1 p.Gly427Cys Two studies have proposed that the Walker-A cysteine residues can be cross-linked to the opposite Walker-A motif [73,74], either through a direct disulphide between the two naturally occurring Walker-A cysteines [74] or between the Walker-A cysteine of NBD2 and a G427C Walker-A NBD1 mutation [73]. Login to comment 681 ABCB1 p.Leu1176Cys More recently, in the light of the NBD sandwich dimer, Loo et al. have demonstrated that the Walker-A cysteine can be cross-linked to a cysteine residue introduced within the opposite signature motif (L1176C, [19]), 10 Å apart (Cα-Cα, Table S1). Login to comment