ABCMdb

PMID: 17574245

Matsumura Y, Sakai H, Sasaki M, Ban N, Inagaki N
ABCA3-mediated choline-phospholipids uptake into intracellular vesicles in A549 cells.
FEBS Lett. 2007 Jul 10;581(17):3139-44. Epub 2007 Jun 6., [PubMed]

Sentences

No. Mutations Sentence Comment

21 ABCA3 p.Asn568Asp Establishment of stable cell line A549 cells stably expressing GFP-tagged Walker A motif mutant (N568D) ABCA3 protein (ABCA3-GFP) were established as previously described [10]. Login to comment 54 ABCA3 p.Asn568Asp Results 3.1. Expression of wild-type ABCA3-GFP enlarges LAMP3-positive vesicles in A549 cells We previously established lung adenocarcinoma A549/ABCA3WT cells, which stably express GFP-tagged wild-type Fig. 1. Expression of wild-type and N568D ABCA3-GFP in A549 cells. Login to comment 55 ABCA3 p.Asn568Asp Results 3.1. Expression of wild-type ABCA3-GFP enlarges LAMP3-positive vesicles in A549 cells We previously established lung adenocarcinoma A549/ABCA3WT cells, which stably express GFP-tagged wild-type Fig. 1. Expression of wild-type and N568D ABCA3-GFP in A549 cells. Login to comment 62 ABCA3 p.Asn568Asp Similarly, GFP-tagged expression plasmid of ABCA3 containing naturally occurring mutation N568D in the Walker A motif, which exhibits impaired ATP-binding and ATP-hydrolysis activities [10], was transfected in A549 cells to generate A549/ABCA3N568D cells. Login to comment 63 ABCA3 p.Asn568Asp Similarly, GFP-tagged expression plasmid of ABCA3 containing naturally occurring mutation N568D in the Walker A motif, which exhibits impaired ATP-binding and ATP-hydrolysis activities [10], was transfected in A549 cells to generate A549/ABCA3N568D cells. Login to comment 104 ABCA3 p.Asn568Asp Discussion In this study, to clarify the mechanism of lipid transport mediated by ABCA3, GFP-tagged wild-type and N568D mutant ABCA3, which exhibits impaired ATP-binding and ATP-hydrolysis activities [10], were stably expressed in A549 Fig. 3. Login to comment 105 ABCA3 p.Asn568Asp Discussion In this study, to clarify the mechanism of lipid transport mediated by ABCA3, GFP-tagged wild-type and N568D mutant ABCA3, which exhibits impaired ATP-binding and ATP-hydrolysis activities [10], were stably expressed in A549 Fig. 3. Login to comment