ABCMdb

PMID: 17561960

de Wet H, Mikhailov MV, Fotinou C, Dreger M, Craig TJ, Venien-Bryan C, Ashcroft FM
Studies of the ATPase activity of the ABC protein SUR1.
FEBS J. 2007 Jul;274(14):3532-44. Epub 2007 Jun 11., [PubMed]

Sentences

No. Mutations Sentence Comment

12 ABCC8 p.Lys719Ala Mutation of the Walker A lysine in nucleotide-binding domain 1 (K719A) or nucleotide-binding domain 2 (K1385M) inhibited the ATPase activity of sulfonylurea receptor 1 by 60% and 80%, respectively. Login to comment 77 ABCC8 p.Lys719Ala Mutating the WA lysine in NBD1 of SUR1 to alanine (K719A) reduced ATPase activity by approximately 60%, whereas mutating the WA lysine in NBD2 to methionine (K1385M) inhibited ATPase activity by about 80% (Fig. 2C) when compared to wild-type controls assayed in parallel (n ¼ 2). Login to comment 87 ABCC8 p.Lys719Ala (C) ATPase activity of SUR1 containing the mutations K719A (triangles, n ¼ 2) or K1385M (diamonds, n ¼ 2). Login to comment 162 ABCC8 p.Lys719Ala Mutation of the WA lysines (K719A, K1385M) reduced ATPase activity by 70-80%. Login to comment