ABCMdb

PMID: 17244610

Papadopoulos M, Momburg F
Multiple residues in the transmembrane helix and connecting peptide of mouse tapasin stabilize the transporter associated with the antigen-processing TAP2 subunit.
J Biol Chem. 2007 Mar 30;282(13):9401-10. Epub 2007 Jan 23., [PubMed]

Sentences

No. Mutations Sentence Comment

39 ABCB2 p.Lys463Ala To generate the ER retrieval signal mutant K463A/K464A of mTpn, the reverse primer 5Ј-GGCCAAGCTTATTGGGAAGCGG- CTGAGTTCCTGGGAATGGTCAGGCTGGC-3Ј was used together with the same forward primer as above and inserted into mTpn cDNA using XbaI and HindIII. Login to comment 87 ABCB2 p.Lys463Ala Also, the mTpn[K463A/K464A] mutant was able to substitute WT mTpn, suggesting that ER retrieval/retention is not essential for the TAP2-stabilizing function of Tpn. Login to comment 237 ABCB2 p.Lys463Ala Since the mutant mTpn[K462A/K463A] was fully functional in terms of TAP2 stabilization, it is conceivable that Tpn acts on TAP in the ER proper and also within the recycling pathway. Login to comment 238 ABCB2 p.Lys463Ala Since the mutant mTpn[K462A/K463A] was fully functional in terms of TAP2 stabilization, it is conceivable that Tpn acts on TAP in the ER proper and also within the recycling pathway. Login to comment