ABCMdb

PMID: 17206937

Kypreos KE, Zannis VI
Pathway of biogenesis of apolipoprotein E-containing HDL in vivo with the participation of ABCA1 and LCAT.
Biochem J. 2007 Apr 15;403(2):359-67., [PubMed]

Sentences

No. Mutations Sentence Comment

29 ABCA1 p.Leu268Ala It is important to note that infection of apoA-I-/- mice with adenovirus expressing a recently described apoE4 (L261A/ W264A/F265A/L268A/V269A) mutant designated apoE4mut1 [17] did not induce dyslipidaemia and promoted the formation of spherical apoE-containing HDL particles, suggesting that this mutant has improved functions in lipoprotein clearance as well as in the biogenesis of apoE-containing HDL. Login to comment 30 ABCA1 p.Leu268Ala It is important to note that infection of apoA-I-/- mice with adenovirus expressing a recently described apoE4 (L261A/ W264A/F265A/L268A/V269A) mutant designated apoE4mut1 [17] did not induce dyslipidaemia and promoted the formation of spherical apoE-containing HDL particles, suggesting that this mutant has improved functions in lipoprotein clearance as well as in the biogenesis of apoE-containing HDL. Login to comment 115 ABCA1 p.Leu268Ala Substitution of alanine for hydrophobic residues in the region 261-269 enhances formation of spherical HDL and does not induce dyslipidaemia in apoA-I-/- mice In another set of experiments, apoA-I-/- mice were infected with 2 &#d7; 109 pfu of an adenovirus expressing the apoE4 mutant (L261A/W264A/F265A/L268A/V269A) mutant designated apoE4mut1 [17]. Login to comment 117 ABCA1 p.Leu268Ala Substitution of alanine for hydrophobic residues in the region 261-269 enhances formation of spherical HDL and does not induce dyslipidaemia in apoA-I-/- mice In another set of experiments, apoA-I-/- mice were infected with 2 × 109 pfu of an adenovirus expressing the apoE4 mutant (L261A/W264A/F265A/L268A/V269A) mutant designated apoE4mut1 [17]. Login to comment 135 ABCA1 p.Leu268Ala These experiments indicated that, under conditions of apoE overexpression, the endogenous Figure 4 Cholesterol FPLC profile, apoE distribution and electron microscopy analysis of HDL fraction of apoE-/- mice infected with recombinant adenoviruses expressing apoE4mut1 (A) FPLC profiles of total, free and esterified cholesterol of apoE-/- mice infected with 2 &#d7; 109 pfu of adenoviruses expressing apoE4mut1 (AdGFRP-E4[L261A,T264A,F265A, L268A,V269A]). Login to comment 137 ABCA1 p.Leu268Ala These experiments indicated that, under conditions of apoE overexpression, the endogenous Figure 4 Cholesterol FPLC profile, apoE distribution and electron microscopy analysis of HDL fraction of apoE-/- mice infected with recombinant adenoviruses expressing apoE4mut1 (A) FPLC profiles of total, free and esterified cholesterol of apoE-/- mice infected with 2 × 109 pfu of adenoviruses expressing apoE4mut1 (AdGFRP-E4[L261A,T264A,F265A, L268A,V269A]). Login to comment