ABCMdb

PMID: 17055487

Muller M, Klein I, Kopacsi S, Remaley AT, Rajnavolgyi E, Sarkadi B, Varadi A
Co-expression of human ABCG5 and ABCG8 in insect cells generates an androstan stimulated membrane ATPase activity.
FEBS Lett. 2006 Nov 13;580(26):6139-44. Epub 2006 Oct 16., [PubMed]

Sentences

No. Mutations Sentence Comment

57 ABCG5 p.Lys92Met Co-expression of the wtABCG5 and wtABCG8 as well as K92M-ABCG5 and wtABCG8 was carried out by using different ratios of the corresponding recombinant baculovirus supernatants. Login to comment 58 ABCG5 p.Lys92Met Co-expression of the wtABCG5 and wtABCG8 as well as K92M-ABCG5 and wtABCG8 was carried out by using different ratios of the corresponding recombinant baculovirus supernatants. Login to comment 95 ABCG5 p.Lys92Met As a negative control, we used the co-expression of a Walker A mutant (ABCG5-K92M) of ABCG5 with the wild-type ABCG8 (G5K92M/G8). Login to comment 96 ABCG5 p.Lys92Met As a negative control, we used the co-expression of a Walker A mutant (ABCG5-K92M) of ABCG5 with the wild-type ABCG8 (G5K92M/G8). Login to comment 118 ABCG5 p.Lys92Met The specific, androgen-stimulated ATPase activity of the ABCG5/ABCG8 heterodimer was abolished when a key lysine was changed to methionine (K92M) in the Walker A motif of ABCG5. Login to comment 119 ABCG5 p.Lys92Met The specific, androgen-stimulated ATPase activity of the ABCG5/ABCG8 heterodimer was abolished when a key lysine was changed to methionine (K92M) in the Walker A motif of ABCG5. Login to comment