ABCMdb

PMID: 16951376

Celli L, Ryckewaert JJ, Delachanal E, Duperray A
Evidence of a functional role for interaction between ICAM-1 and nonmuscle alpha-actinins in leukocyte diapedesis.
J Immunol. 2006 Sep 15;177(6):4113-21., [PubMed]

Sentences

No. Mutations Sentence Comment

73 ABCC8 p.Arg486Ala ICAM-1 cytoplasmic mutants: CHO ICAM-1 R480A, K481A, K483A, K484A, and R486A were generated using the QuickChange Site-Directed Mutagenesis kit (Stratagene). Login to comment 197 ABCC8 p.Arg486Ala In this study, we compared the interaction level between ICAM-1, considered as positive control, and each mutant (ICAM-1 R480A, K481A, K483A, K484A, and R486A) with both ␣-actinin 1 and 4. Login to comment 247 ABCC8 p.Arg486Ala All the rCHO cell lines expressing ICAM-1, ICAM-1⌬Cyt, and mutants (R480A, K481A, K483A, K484A, and R486A) formed monolayers that were nearly impermeable to HRP, with 12.0 Ϯ 3.9%, 9.1 Ϯ 0.8%, 9.8 Ϯ 1.9%, 10.1 Ϯ 5.6%, 16.4 Ϯ 1.9%, 5.7 Ϯ 0.6%, and 12.2 Ϯ 3.3% of controls, respectively; these values are similar to those obtained with WT CHO monolayers (11.9 Ϯ 1.1%) (Fig. 6). Login to comment 251 ABCC8 p.Arg486Ala As shown in Fig. 7, when ICAM-1/␣-actinin interactions were altered, the number of neutrophils transmigrating across CHO monolayers was reduced to a level similar to CHO WT or CHO ICAM-1⌬Cyt (14.22 Ϯ 3.32% for ICAM-1 R 480A, 14.92 Ϯ 5.25% for ICAM-1 K481A, and 16.2 Ϯ 3.54% for ICAM-1 R486A vs 13.33 Ϯ 2.17% for CHO WT and 14.94 Ϯ FIGURE 5. Login to comment 336 ABCC8 p.Arg486Ala Given the similar effect of ICAM-1 mutants R480A, K481A, and R486A on both ␣-actinin 1 and 4 interaction, we were not able to conclude which isoform of ␣-actinins was involved in transmigration. Login to comment