ABCMdb

PMID: 16889754

Wan CK, Zhu GY, Shen XL, Chattopadhyay A, Dey S, Fong WF
Gomisin A alters substrate interaction and reverses P-glycoprotein-mediated multidrug resistance in HepG2-DR cells.
Biochem Pharmacol. 2006 Sep 28;72(7):824-37. Epub 2006 Aug 4., [PubMed]

Sentences

No. Mutations Sentence Comment

112 ABCB1 p.Phe983Ala ABCB1 p.Phe983Ala Baculovirus mediated expression of human Pgp and photoaffinity labeling of Pgp with [125 I]IAAP Recombinant baculoviruses harboring either the wild type BV-MDR1-(H6) [24] or the F983A mutant BV-MDR1-F983A- (H6)[25] of human MDR1 cDNA, with 6 His-tag at the C-terminal end, were used to infect High Five insect cells grown in serum free Excell 400 medium as described [26]. Login to comment 113 ABCB1 p.Phe983Ala ABCB1 p.Phe983Ala Baculovirus mediated expression of human Pgp and photoaffinity labeling of Pgp with [125 I]IAAP Recombinant baculoviruses harboring either the wild type BV-MDR1-(H6) [24] or the F983A mutant BV-MDR1-F983A- (H6)[25] of human MDR1 cDNA, with 6 Â His-tag at the C-terminal end, were used to infect High Five insect cells grown in serum free Excell 400 medium as described [26]. Login to comment 216 ABCB1 p.Phe983Ala To understand whether F983 has any role in the interaction of gomisin A with Pgp, we studied [125 I]IAAP labeling of Pgp F983A mutant in the presence of varying concentrations of gomisin A. Login to comment 217 ABCB1 p.Phe983Ala To understand whether F983 has any role in the interaction of gomisin A with Pgp, we studied [125 I]IAAP labeling of Pgp F983A mutant in the presence of varying concentrations of gomisin A. Login to comment 237 ABCB1 p.Phe983Ala b i o c h e m i c a l p h a r m a c o l o g y 7 2 ( 2 0 0 6 ) 8 2 4 - 8 3 7 835 Fig. 10 - Effect of gomisin A on [125 I]IAAP photo-labeling of the wild type and F983A mutant Pgp. Login to comment 238 ABCB1 p.Phe983Ala ABCB1 p.Phe983Ala Wild type (Fig. 10A, top panel) or F983A mutant (Fig. 10B, bottom panel) Pgp in isolated membranes was photoaffinity labeled with 5 nM of [125 I]IAAP in the presence of varying concentrations (0-100 mM) of gomisin A (GOM). Login to comment 239 ABCB1 p.Phe983Ala Wild type (Fig. 10A, top panel) or F983A mutant (Fig. 10B, bottom panel) Pgp in isolated membranes was photoaffinity labeled with 5 nM of [125 I]IAAP in the presence of varying concentrations (0-100 mM) of gomisin A (GOM). Login to comment 246 ABCB1 p.Phe983Ala However, gomisin A`s effect on [125 I]IAAP labeling of Pgp was not altered in the Pgp F983A mutant that is impaired of modulation by cis- (Z)-flupentixol. Login to comment 247 ABCB1 p.Phe983Ala However, gomisin A`s effect on [125 I]IAAP labeling of Pgp was not altered in the Pgp F983A mutant that is impaired of modulation by cis- (Z)-flupentixol. Login to comment