ABCMdb

PMID: 16804061

Stalvey MS, Muller C, Schatz DA, Wasserfall CH, Campbell-Thompson ML, Theriaque DW, Flotte TR, Atkinson MA
Cystic fibrosis transmembrane conductance regulator deficiency exacerbates islet cell dysfunction after beta-cell injury.
Diabetes. 2006 Jul;55(7):1939-45., [PubMed]

Sentences

No. Mutations Sentence Comment

38 ABCC7 p.Ser489* RESEARCH DESIGN AND METHODS We used mice with the CFTR S489X-/- neo insertion, developed initially at the University of North Carolina (20). Login to comment 42 ABCC7 p.Ser489* CFTR S489X-/- FABP-hCFTRϩ/ϩ (hereafter designated CFTR-/- ), C57BL/6J, and FVB/NJ mice were housed under specific pathogen-free conditions at the University of Florida Animal Care Services according to National Institutes of Health guidelines and allowed food and water ad libitum. Login to comment 56 ABCC7 p.Ser489* CFTR S489X-/- animals (4-6 weeks old) were sensitized to Aspergillus fumigatus crude protein extract (Greer Laboratories, Lenoir, NC). Login to comment 66 ABCC7 p.Ser489* Lung, intestine, and pancreatic mRNA from CFTR S489X-/- FABP-hCFTR؉/؉ mice was used to analyze the expression pattern of the hCFTR driven by the FABP promoter by RT-PCR, using gene-specific primers for hCFTR and B-actin control primers. Login to comment 180 ABCC7 p.Ser489* Interestingly, the CFTR S489X-/- FABP-hCFTRϩ/ϩ model used in this experiment has not been previously reported to have pancreatic disease. Login to comment