ABCMdb

PMID: 16781659

Makkar RS, Contreras MA, Paintlia AS, Smith BT, Haq E, Singh I
Molecular organization of peroxisomal enzymes: protein-protein interactions in the membrane and in the matrix.
Arch Biochem Biophys. 2006 Jul 15;451(2):128-40. Epub 2006 May 24., [PubMed]

Sentences

No. Mutations Sentence Comment

91 ABCD1 p.Arg113Pro ABCD1 p.Arg113Pro Two peptides (sequences L1: 104 RTFLSVYVARLDGRL ARCIVRKDPRA and L1m: same sequence as L1 but carrying R113P mutation; Arg to Pro at position 113) [25] were custom synthesized with a purity of 98% or higher by Sigma-Genosys (The Woodlands, TX, USA). Login to comment 158 ABCD1 p.Arg113Pro In parallel, a second synthetic peptide with the same amino acid sequence but carrying a single amino acid substitution (R113P) was used for the same analysis (L1m: 104 RTFLSVYVAPLDGRLARCIVRKDP RA). Login to comment 165 ABCD1 p.Arg113Pro ABCD1 p.Arg113Pro The sequence of the synthetic peptides L1 and L1m were similar (104 RTFLSVYVARLDGRLARCIVRKDPRA), but L1m carry a R113P mutation (Arg to Pro at position 113). Login to comment 166 ABCD1 p.Arg113Pro ABCD1 p.Arg113Pro ABCD1 p.Arg113Pro The sequence of the synthetic peptides L1 and L1m were similar (104 RTFLSVYVARLDGRLARCIVRKDPRA), but L1m carry a R113P mutation (Arg to Pro at position 113). Login to comment 167 ABCD1 p.Arg113Pro Peptide KD L1 3.3 £ 10¡8 6.0 £ 10¡8 L1m (R113P) 1.1 £ 10¡7 1.6 £ 10¡7 techniques (yeast two-hybrid system and SPR) indicate that ALDP and VLCS physically interact in the peroxisome membrane. Login to comment