ABCMdb

PMID: 16680030

Krimbou L, Marcil M, Genest J
New insights into the biogenesis of human high-density lipoproteins.
Curr Opin Lipidol. 2006 Jun;17(3):258-67., [PubMed]

Sentences

No. Mutations Sentence Comment

55 ABCA1 p.Cys1477Arg We have previously shown [40], however, that incubation of lipid-free apoE3 with normal fibroblasts generated nascent apoE3/cholesterol/phospholipid complexes that exhibited preb-electrophoretic mobility with a particle size ranging from 9 to 15 nm, whereas lipid-free apoE3 incubated with ABCA1 mutant (C1477R) cells was unable to form such particles. Login to comment 63 ABCA1 p.Gln597Arg ABCA1 p.Cys1477Arg We have previously suggested that the high content in phosphatidylinositol [41] or the high number of apoA-I molecules per particle [43] (Fig. 2b, lower panels) may contribute to the increase in the net negative charge of nascent LpA-I and consequently cause 260 Lipid metabolism Table 1 Formation and speciation of nascent apolipoprotein (apo)A-I-containing particles in various cell lines Cell type Pre-b1-LpA-I a-LpA-I Macrophages monocyte-derived þ þ THP-1 þ þ Hepatocytes HepG2 (apoA-I endo, exo) þ þ primary mouse (apoA-I endo) þ þ CaCo-2 (apoA-I endo, exo) - þ Fibroblasts normal - þ Tangier (Q597R) - - Tangier (C1477R) - - HUVEC - - CHO - þ CHO overexpressing ABCA1 - þ The presence of pre-b1-LpA-I and a-LpA-I generated by different cell lines was determined by two-dimensional polyacrylamide nondenaturing gradient gel electrophoresis based on our previous study [28]. Login to comment