ABCMdb

PMID: 16675347

Saadane A, Soltys J, Berger M
Acute Pseudomonas challenge in cystic fibrosis mice causes prolonged nuclear factor-kappa B activation, cytokine secretion, and persistent lung inflammation.
J Allergy Clin Immunol. 2006 May;117(5):1163-9., [PubMed]

Sentences

No. Mutations Sentence Comment

28 ABCC7 p.Ser489* METHODS Mice Male CF-KO mice 7 to 10 weeks old homozygous for the S489X (B6.129P2-CftrtmtUnc ) (null) mutation17 and normal wt/wt (WT) littermates were used after genotyping by polymerase chain reaction on tail snip DNA. Pups were weaned at 10 days of life and fed Peptamen (Clintech Nutrition Co, Deerfield, Ill) to avoid intestinal obstruction.18 All mice were maintained in sterile microisolator cages with sterile water. Login to comment 140 ABCC7 p.Gly551Asp Two other studies, using mice with the G551D mutation in CFTR, also showed that those mice display an abnormal inflammatory response with increased cytokines and infiltrating cells.24,25 Because the genes for proinflammatory cytokines, such as TNF, IL-1b, and IL-6, are activated by NF-kB,22 we studied NF-kB activation in the lung tissue. Login to comment