ABCMdb

PMID: 16621797

Vij N, Fang S, Zeitlin PL
Selective inhibition of endoplasmic reticulum-associated degradation rescues DeltaF508-cystic fibrosis transmembrane regulator and suppresses interleukin-8 levels: therapeutic implications.
J Biol Chem. 2006 Jun 23;281(25):17369-78. Epub 2006 Apr 18., 2006-06-23 [PubMed]

Sentences

No. Mutations Sentence Comment

57 ABCC7 p.Trp1282* ABCC7 p.Trp1282* Cell Culture, Transfection, and Metabolic Labeling-IB3-1 cells (⌬F508/W1282X, low level expression of ⌬F508-CFTR and no W1282X-CFTR protein), CF tracheal epithelial (CFTE) cells (homozygous for ⌬F508-CFTR), and S9 cells (IB3-1 cells corrected with adeno-associated virus-CFTR) were maintained in LHC-8 medium (BIOSOURCE) containing 100 units/ml penicillin, 100 ␮g/ml streptomycin, 0.25 ␮g/ml amphotericin B, and 10% fetal bovine serum (all from Invitrogen). Login to comment 110 ABCC7 p.Trp1282* The efficiency of VCP shRNA (⌬VCP)-mediated inhibition of VCP protein levels in IB3-1 cells (⌬F508/W1282X CF bronchial epithelial line) (13) was evaluated by immunoblotting of whole cell lysates (Fig. 1B, upper panel). Login to comment