PMID: 16512671

Singh OV, Vij N, Mogayzel PJ Jr, Jozwik C, Pollard HB, Zeitlin PL
Pharmacoproteomics of 4-phenylbutyrate-treated IB3-1 cystic fibrosis bronchial epithelial cells.
J Proteome Res. 2006 Mar;5(3):562-71., [PubMed]
Sentences
No. Mutations Sentence Comment
21 ABCC7 p.Trp1282*
X
ABCC7 p.Trp1282* 16512671:21:26
status: NEW
view ABCC7 p.Trp1282* details
 IB3-1 cells (∆F508/W1282X CF bronchial epithelial cells)22 were grown on uncoated 75 cm2 tissue culture flasks (Falcon, Franklin Lakes, NJ) in a 5% CO2 incubator at 37 °C for 48-72 h to reach 80% confluency. Login to comment 76 ABCC7 p.Trp1282*
X
ABCC7 p.Trp1282* 16512671:76:99
status: NEW
view ABCC7 p.Trp1282* details
 We studied the immortalized CF bronchial epithelial cell line IB3-1,22 (CFTR genotype ∆F508/W1282X). Login to comment 77 ABCC7 p.Trp1282*
X
ABCC7 p.Trp1282* 16512671:77:64
status: NEW
view ABCC7 p.Trp1282* details
 IB3-1 expresses the ∆F508 CFTR protein only, because the W1282X mutation produces an unstable, and therefore untranslated, mRNA.26,27 We have previously published the dose response curve for 4-PBA mediated rescue of ∆F508 CFTR processing in IB3-1 cells. Login to comment